DNA Bisulfite Conversion Kit Featured Image

I-DNA Bisulfite Conversion Kit

Ukuguqulwa nokuhlanzwa kungaqedwa ngehora eli-2, ngezinga lokuguqulwa lifike kuma-99%.

I-DNA Bisulfite Conversion Kit yenzelwe ngokukhethekile ukuguqulwa kwe-DNA bisulfite ocwaningweni lwe-DNA methylation. Ukwelashwa kwamasampuli e-DNA kusetshenziswa le khithi kungaqedwa emahoreni ama-2, futhi izinga lokuguqulwa kwe-cytosine engashintshi ibe yi-uracil ingaphezu kwama-99%. Leli khithi lamukela ingxenye eyingqayizivele ye-ejenti yokuvikela ye-DNA, eqinisekisa ikhwalithi nokuvuselelwa kwe-DNA ngemuva kokuguqulwa. Ngaphezu kwalokho, le kit iphinda isebenzise indlela yokususwa kweqembu le-sulphurous acid group, okwenza yonke inqubo yokusebenza ibe lula.

Ikati. Cha	Ukupakisha Usayizi
4992447	Ama-preps angama-50

Thumela i-imeyili kithi

Imininingwane yomkhiqizo

Isibonelo sokuhlola

Amathegi womkhiqizo

Izici

■ Kulula futhi kuyashesha: Inqubo yokuguqula nokuhlanza ingaqedwa ngamahora ama-2, ngezidingo eziphansi zezinsimbi nemishini, efanele izikhungo zocwaningo kuwo wonke amazinga.
■ Izinga lokuguqulwa eliphakeme: Izinga lokuguqulwa kwe-cytosine engashintshiwe ibe yi-uracil kusampula ye-DNA ingaphezu kwama-99%.
■ Ukuzwela okuphezulu: Le khithi ingacubungula amasampula e-DNA angafika ku-500 pg aze afike ku-2.5 μg.

Ukucaciswa

Uhlobo: Ukuguqulwa kweBisulfite
Isampula ye-DNA: 500 pg-2.5 μg
Izinga lokuguqulwa: > 99%
Isikhathi sokusebenza: ~ 2 ihora
Izicelo: Amasampula e-DNA acutshungulwa yileli kit afanele i-PCR / qPCR ethile ye-methylation, ukulandelana kanye ne-microarray.

Yonke imikhiqizo ingenziwa ngezifiso i-ODM / OEM. Ngemininingwane,sicela uchofoze i-Customized Service (ODM / OEM)

Langaphambilini I-TIANSeq mRNA Capture Kit

Olandelayo: I-Methylation-specipc PCR (MSP) Kit

Ithebula 1: Izinga lokuguqulwa kwe- “C” elingashintshiwe elithi “U”
Kusetshenziswa izakhi zofuzo zomuntu njengezinto zokuhlola, isilinganiso sokutakula sesampula singafinyelela kuma-65%, futhi isilinganiso se- “C” esingashintshiwe ku- “U” singafinyelela ngaphezu kuka-99%.

Table 1: Conversion rate of unmethylated “C” to “U” Using human genome as experimental material, the recovery rate of the sample can reach 65%, and the ratio of unmethylated “C” to “U” can reach more than 99%.