I-EndoFree Maxi Plasmid Kit V2

Ukuhlanzwa kwe-endotoxin-free transfection grade plasmid DNA eqondene namaseli abucayi.

I-EndoFree Maxi Plasmid Kit V2 isebenzisa inqubo eyingqayizivele ye-silica membrane adsorption ukuze ibophe kahle futhi ibophele i-DNA ye-plasmid. IBuffer ER ekhethekile kanye neFiltration CS1 kungasusa ngempumelelo ukungcola okufana ne-endotoxin namaprotheni. Yonke inqubo yokukhipha ithatha ihora elilodwa kuphela.
Kunconywe inani eliphakathi nendawo lesiko lamagciwane: Nge-plasmid enekhophi ephezulu, kunconywa ibhaktheriya elingu-100 ml, elinganikela ku-500-1500 μg plasmid. Nge-plasmid enekhophi ephansi, 200 ml yamabhaktheriya aphakathi anconyelwa ukufeza isivuno cishe esingu-50-300 μg.

Ikati. Cha	Ukupakisha Usayizi
4992438	Ama-preps ayi-10

Thumela i-imeyili kithi

Imininingwane yomkhiqizo

Ukuhamba komsebenzi

Isibonelo sokuhlola

Amathegi womkhiqizo

Izici

■ Ukuhlanzeka okuphezulu: Ubuchwepheshe obuyingqayizivele bemvula ye-endotoxin buthathwa ukuze kususwe ngokuqondile i-endotoxin.
■ Kulula ukusebenza: Ubuchwepheshe bekholomu ye-adsorption busetshenziselwa ngqo ukukhangisa i-plasmid DNA, eyenza ukusebenza kube lula.
■ Ukudluliswa kokusebenza kahle okuphezulu: Kulungele ukwedluliswa kolayini abaningi bamaseli kubandakanya amaseli anozwela i-endotoxin.
■ Izicelo ezahlukahlukene: I-plasmid ehlanziwe ingasetshenziswa ekudlulisweni kwamangqamuzana ezilwane nezitshalo kanye nasekuhlolweni kwe-biology yamangqamuzana.

Izicelo

I-plasmid DNA ekhishwe kusetshenziswa le khithi ingasetshenziselwa imisebenzi eyahlukahlukene ejwayelekile, kufaka phakathi ukuvinjelwa kwe-enzyme digestion, i-PCR, ukulandelana, i-ligation, ukuguqulwa kanye nokuhanjiswa kwamaseli ahlukahlukene.

Yonke imikhiqizo ingenziwa ngezifiso i-ODM / OEM. Ngemininingwane,sicela uchofoze i-Customized Service (ODM / OEM)

Langaphambilini I-TIANprep N96 Magnetic Plasmid Kit

Olandelayo: I-STIANqucik N96 Kit Yokuhlanzwa

I-Buffer ER eyingqayizivele yokususa i-endotoxin ingasusa kahle izinsalela ze-endotoxin ohlelweni lokuphendula, futhi i-plasmid ephezulu yokuhlanzeka ingatholakala. Izinsalela ze-plasmid endotoxin ngu- ≤ 0.1 EU / ml.

I-plasmid ihlanzwe kusetshenziswa i-EndoFree Maxi Plasmid Kit V2 kanye nemikhiqizo efanayo evela ku-Supplier 1 naku-Supplier 2 ihlanganiswe nomthamo ofanayo we-elution buffer. I-1 μl plasmid yalayishwa kulayini ngamunye ukulinganisa ukugxila kweplasmid. I-100 ng ye-plasmid okugxilwe kuyo okwakunqunywa yi-spectrophotometer kwalayishwa ku-gel efanayo ukuthola ukuthi inani lokuhlushwa laliphezulu yini ngokukhohlisa.
Isiphetho: Umphumela we-electrophoresis ukhombisa ukugxila kwangempela kweplasmid ekhishwe i-EndoFree Maxi Plasmid kit V2, noma ngabe ikhophi ephezulu noma ikhophi ephansi, iphezulu kakhulu kunaleyo ekhishwe abahlinzeki 1 no-2.

Bhala umyalezo wakho lapha bese uwuthumela kithi