Ikhithi ye-EndoFree Midi Plasmid

Ukuhlanzwa okusheshayo kwenani eliphakathi le-endotoxin-free transfection grade plasmid DNA.

I-EndoFree Midi Plasmid Kit isebenzisa inqubo eyingqayizivele ye-silica membrane adsorption ukuze ibophe kahle futhi ibophele i-DNA ye-plasmid. I-Buffer EBT ekhethekile ne-Filtration CS1 kungasusa ngempumelelo ukungcola okufana ne-endotoxin namaprotheni. Yonke inqubo yokukhipha ithatha ihora elilodwa kuphela, elilula futhi elisheshayo.

Ikati. Cha	Ukupakisha Usayizi
4992853	Ama-preps ayi-10

Thumela i-imeyili kithi

Imininingwane yomkhiqizo

Ukuhamba komsebenzi

Isibonelo sokuhlola

Amathegi womkhiqizo

Izici

S Ilungele ukukhipha ama-plasmid ku-50-100 ml yamasiko aphakathi kwamasiko.
■ I-plasmid ehlanziwe iqukethe i-endotoxin ephansi kakhulu futhi ingahlangabezana nezidingo zokudluliswa kwekhwalithi ephezulu.
■ Kulula ukusebenza ngenqubo yokususa i-endotoxin eyingqayizivele futhi esheshayo.

Izicelo

I-plasmid DNA ehlanziwe ingasetshenziswa ekuhlolweni kwe-biology yamangqamuzana kanye nokuhlolwa kwe-cell biology njengokuvinjelwa kwe-endonuclease digestion, ukulandelana, ukuguqulwa, ukuguqulwa, i-microinjection, njll.

Isivuno sePlasmid

Yonke imikhiqizo ingenziwa ngezifiso i-ODM / OEM. Ngemininingwane,sicela uchofoze i-Customized Service (ODM / OEM)

Langaphambilini I-TIANprep Rapid Mini Plasmid Kit

Olandelayo: I-TIANprep Rapid N96 Plasmid Kit

	I-Plasmid pEGFP-C1 ihlanjululwa yi-EndoFree Midi Plasmid Kit kusuka ku-50 ml ngobusuku obuthile obuphakathi kwamagciwane. Ukuhlungwa kweplasmid kukalwe yiNanodrop futhi isivuno sabalwa. Umphumela ukhombisa ukuthi isivuno se-plasmid nge-EndoFree Mini Plasmid Kit siphakeme kunalokho ngomkhiqizo ofanele ovela kuMhlinzeki A.
	I-plasmid pEGFP-C1 ekhishwe yi-EndoFree Midi Plasmid Kit idluliselwe kuma-80% confluence 293T cell yi-TIANGEN TIANfect. Ukuchazwa kwe-GFP kutholakale i-24-36 h ngemuva kokufakwa emzimbeni. Umphumela ukhombisa ukuthi ukusebenza kahle kokuhanjiswa kwakungu-80% -90%.