Ikhithi ye-FastKing One Step RT-qPCR

Isinyathelo esisodwa se-Real-Time RT-PCR ene-SYBR-Green.

I-FastKing One Step RT-qPCR Kit yenzelwe i-Real-Time One Step RT-qPCR ngokusebenzisa i-SYBR Green I. Ikhithi ingasetshenziswa ngesikhathi sangempela sesinyathelo esisodwa i-RT-qPCR yezinhloso ze-RNA, ngokubhalwa phansi kwe-reverse ne-PCR kwenzeka ngokulandelana kushubhu efanayo. Ngakho-ke ukusebenza kulula, futhi kunciphisa ubungozi bokungcola.

I-25 × RT-PCR Enzyme Mix kule kit iyisiqalo se-TIANGEN's novel reverse transcriptase (FastKing RTase), i-anti-anti-modified hotstart Isi-Taq I-DNA polymerase ne-RNase Inhibitor. I-FastKing RTase wuhlobo olusha lwe-molecular modified reverse transcriptase. Ngesici se-hydrophobic esingezwe ngokukhethekile, esinobuhlobo obuqinile be-RNA nokuqina kokushisa. I-FastKing RTase ithuthukise ukusebenza kahle kokubhalwa phansi okuguquguqukayo kanye nekhono lokunweba ithempulethi ye-RNA ngesakhiwo esiyinkimbinkimbi sesibili. Uhlobo olusha lwe-hotstartIsi-Taq I-DNA polymerase esebenza kahle kakhulu isetshenziswa kwinqubo ye-PCR, okwenza ukusabela kwe-PCR ngemuva kokubhalwa phansi kwe-reverse kube nokusebenza okuphezulu kokukhulisa nokucaciswa. Ngaphezu kwalokho, i-2 × FastKing RT-qPCR Buffer (SYBR Green) kulo mkhiqizo uhlelo olusha lokuphendula olwenzelwe ngokukhethekile ama-enzyme amabili angenhla, kufaka phakathi izinto ezidingekayo ze-ionic, ama-dNTP, udayi ohlaza okotshani we-SYBR, isinyathelo esisodwa sokuqinisa kanye nesithuthukisi, ingaqinisekisa ukuthi i-FastKing RTase ne-hotstart entshaIsi-Taq I-DNA polymerase ingadlala indima ephumelela kunazo zonke enqubweni eyodwa yokuphendula okukodwa.

Le kit inikeza ama-curve ajwayelekile ajwayelekile ezindaweni ezahlukahlukene zokulinganisa. Ingakwazi ukuthola ngokunembile nangokubala izinhlobonhlobo zezakhi zofuzo eziphakeme kakhulu neziphansi ezinokuphindeka okuhle nokwethenjelwa okuphezulu.

Ikati. Cha Ukupakisha Usayizi
4993103 50 ×l × 50 rxn

Imininingwane yomkhiqizo

Isibonelo sokuhlola

Amathegi womkhiqizo

Izici

■ Ukukhuphuka kokusebenza okuphusile: Ikhwalithi ephezulu ye-reverse transcriptase ne-Taq polymerse iqinisekisa ukusebenza kahle kokuphendula okuphezulu.
■ Ukusebenza okulula nokusheshayo: Ifomu lezinto ezimbili lenza inqubo ibe lula futhi isheshe.
■ Ukukwazi Ukufunda ngezifanekiso eziyinkimbinkimbi: Kufanele izifanekiso ze-RNA ezinokuqukethwe okuphezulu kwe-GC nokwakheka okuyinkimbinkimbi kwesekondari.
■ Ukuhambisana okuphezulu: Iyahambisana nezifanekiso ze-RNA ezivela kumthombo wesampula ohlukile noma ngokungcola.

Izicelo

I-RT-qPCR ingasetshenziselwa ukuthola ukubonakaliswa kofuzo kanye nokuqukethwe kwegciwane le-RNA kumaseli nakwizicubu.

Yonke imikhiqizo ingenziwa ngezifiso i-ODM / OEM. Ngemininingwane,sicela uchofoze i-Customized Service (ODM / OEM)


  • Langaphambilini
  • Olandelayo:

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    Izinto zokwakha: I-RNA ephelele yabantu; Isakhi sofuzo esihlosiwe: Uhlobo lwe-β-actin; Ukuhlanzwa kwesifanekiso: Nciphisa isifanekiso ngokuphindaphinda okungu-10 kuye ku-0.001 ng -100 ng; Kit One RT-qPCR Kit: FastKing One Step RT-qPCR Kit (SYBR Green).

    Experimental Example

    Bhala umyalezo wakho lapha bese uwuthumela kithi