NgoDisemba 2019, uchungechunge lwamacala we-pneumonia wesizathu esingaziwa seluqale eWuhan, esifundazweni saseHubei, nase ngokushesha yasakazekela ezifundazweni eziningi nasemadolobheni aseChina, nakwamanye amazwe amaningi ngoJanuwari 2020. Kusukela ngo-22: 00 ntambama ngoJanuwari 27, amacala angama-28 aqinisekisiwe kanye namacala angu-5794 okusolwa ukuthi yi-2019-nCov abikiwe eChina. I-umthombo wokutheleleka kwaleli gciwane ubhekwe njengoRhinolophus, kanti izinga lokufa lamanje lingu-2.9%.
NgoJanuwari 12, 2020, iWorld Health Organisation (WHO) yamemezela ukuthi ubhadane lwe-pneumonia lubangelwe uhlobo olusha lwe-coronavirus (2019-nCov). ICoronavirus uhlobo lwegciwane olusakazeka phakathi kwezilwane. Uhlobo lwegciwane le-nucleic acid luyi-RNA eyodwa. Ngasikhathi sinye, i-WHO iphinde yakhipha imininingwane yokulandelana kwe-nucleic acid ye-nCov eyabiwe izifundiswa zase China, enikeze isisekelo sokutholwa kwamangqamuzana sokutholwa kwegciwane futhi yenze ukutholakala okusheshayo nokuhlonza amagciwane kube lula.
I-WHO iphakamisa ukuthi abantu abanezifo ezinzima zokuphefumula ezifana nokukhwehlela, umkhuhlane, ukutheleleka okuphefumulayo futhi abaye eWuhan kungakapheli izinsuku eziyi-14 noma bevezwe ukuxhumana nezinye iziguli kufanele bahlolwe ngokuphelele. Ngo-17 Januwari 2020, i-WHO yakhipha i- "Laboratory testing for 2019 novel coronavirus (2019-nCoV) emacaleni abantu asolwayo, i-Interim guidance, 17 January 2020". Umhlahlandlela uveza ukuthi izibonelo okufanele ziqoqwe ezigulini ezinesifo zifaka phakathi izibonelo zokuphefumula (i-nasopharynx ne-oropharyngeal swabs, isikhwehlela, ukuhlanza i-bronchoalveolar, njll.) Kanye nezinhlobo ze-serum, ngokulandelayo:
* Ekuthuthweni kwamasampuli okutholwa yi-virus, sebenzisa i-VTM (into yokuhambisa amagciwane) equkethe izithako zokulwa nokubulala amagciwane. Ngokwesiko lamagciwane noma isikhunta, thutha owomile noma ngesilinganiso esincane kakhulu samanzi oyinyumba. Gwema ukubanda okuphindaphindiwe nokuncibilika kwama-specimens.
Ngaphandle kwezinto eziqoqiwe zokuqoqa eziboniswe etafuleni kuqinisekisa nokuthi ezinye izinto zokusebenza nemishini kuyatholakala: isb. Iziqukathi zezokuthutha kanye nezikhwama zokuqoqa izifanekiso kanye nokupakisha, izinto ezipholile namaphakethe abandayo noma iqhwa elomile, imishini yokudweba igazi (isib. Izinaliti, imijovo kanye namashubhu), amalebula nomaka abasebenza unomphela, i-PPE, izinto zokuqeda ukungcoliswa kwendawo.
Ngokuthola igciwane, izinhlobo ze-serum zingasetshenziswa ukuthola i-virus ngendlela ye-immunological, kuyilapho i-RTqPCR inconywa ukuthola i-nCov nucleic acid ukuthola ngokushesha nangokunembile. Njengegciwane
Ukulandelana kuyaziwa, okuqukethwe kwe-viral nucleic acid ku-specimen kungatholakala ngokushesha kuphela
ukukhetha ama-reagents afanelekile nama-primers afanayo
(Ukutholakala kwe-Wuhan coronavirus 2019 nge-real-time RTPCR, Protocol kanye nokuhlola kokuqala kusukela ngoJan 13, 2020) Njengomholi emkhakheni wokukhishwa kwe-nucleic acid nokutholwa eChina, I-TIANGEN BIOTECH (BEIJING) CO., LTD. uhlinzeke ngamagciwane atholakala ngesandla nangemfuluwenza A (H1N1) ama-reagents kubantu abangaphezu kwezigidi eziyi-10. Ku-2019, ama-reagents we-TIANGEN's virus nucleic acid extraction and detection asetshenzisiwe kumasampula we-swine fever yase-Afrika ayizigidi ezingama-30, enza igalelo elivelele ekuhlolweni nasekuvinjelweni kwe-African classical swine fever eChina. I-TIANGEN ayihlinzeki nje kuphela ngama-reagents esheshayo futhi anembile okukhishwa kwamagciwane, kepha futhi ihlinzeka ngezinsimbi zokugaya izicubu ezilula futhi ezisebenza kahle kanye ne-othomathikhi ye-nucleic acid extractors, eyakha isethi ephelele yezixazululo zokukhishwa kwegciwane nokutholwa.
Isixazululo Sokukhipha Isixazululo se-Nucleic Acid
I-TIANGEN Automated Nucleic Acid Extractors yizingxenyekazi ezizenzakalelayo zesizinda se-nucleic acid kusetshenziswa indlela yobuhlalu kazibuthe. Ukusetshenziswa kwalezi zingxenyekazi akugcini nje ngokunciphisa kakhulu umthwalo wemisebenzi yokuhlola nokuhlukaniswa kwabantu, kepha futhi kunciphisa amaphutha okusebenza wokukhipha ngesandla, futhi kuqinisekisa ukuvumelana nokuzinza kwekhwalithi ye-nucleic acid ekhishiwe.
I-TIANGEN Automated Nucleic Acid Extractor inezindlela ezahlukahlukene (kufaka phakathi iziteshi eziyi-16, 24, 32, 48, 96), futhi ama-reagents afanayo angasetshenziselwa ukukhipha i-nucleic acid ezinhlobonhlobo zezinhlobo zesampula. I-TIANGEN iphinde inikeze ngezinsizakalo zokuthuthukisa ezenzelwe kabusha kanye nezinsimbi zokuhlanganisa izinsimbi ngokuya ngezidingo ezihlukile zokuhlola.
I-TGrinder H24 Tissue Homogenizer
● Isetshenziswa kabanzi ekugayeni nasekuhlobiseni izicubu namasampula endle ahlukahlukene
● Ngamandla okugaya i-homogenization izikhathi ezi-2-5 zamathuluzi wendabuko
● Ukugaya okungafaniyo nokuhlanganiswa kwe-homogenization, ukugwema ukungcoliswa okuphambene
● Idivayisi yokuvikela okuzenzakalelayo ukuvikela ukuphepha kwabasebenzi baselebhu
TGuide S32 Automated Nucleic Acid extractor
● Isampula lokukhipha: amasampula 1-32
● Icubungula ivolumu: 20-1000 μl
● Uhlobo lwesampula: Igazi, amaseli, izicubu, indle, igciwane namanye amasampula
● Isikhathi sokucubungula: Kufika kumaminithi ayi-8 ukuthola i-viral nucleic acid
● Imodi yokulawula: Imodi yokulawula okubili ye-Windows Pad nenkinobho yesikrini
Ukuthuthukiswa kwepulatifomu: Ipulatifomu evulekile, ekhululekile ukufanisa ama-reagents
● I-TGuide S32 Magnetic Viral DNA / RNA Kit (DP604)
● Ukusetshenziswa okubanzi: Igciwane le-DNA / i-RNA esezingeni eliphakeme lingahlanzwa kusuka ku-serum, i-plasma, isampula ye-swab, isixazululo sokwelashwa kwezicubu kanye nezixazululo ezahlukahlukene zokulondolozwa kwamagciwane.
● Ilula futhi isebenza kahle: Lo mkhiqizo uhambisana ngokuphelele ne-TGuide S32 Automated Nucleic Acid Extractor, engakhipha igciwane le-DNA / RNA ngokuvunwa okuphezulu, ubumsulwa obuphezulu, ikhwalithi ezinzile nethembekile.
● Izicelo ezisezansi: I-nucleic acid ehlanziwe ilungele ucwaningo lwaphansi lwe-virus PCR kanye ne-PCR yesikhathi sangempela.
Isixazululo Se-Manual Nucleic Acid Extraction
Njengomholi emkhakheni wokukhishwa nokutholwa kwe-nucleic acid, I-TIANGEN inochungechunge oluphelele kakhulu lwemikhiqizo yokukhipha i-nucleic acid eChina, okulungele ukutholwa kwe-coronavirus kuzo zonke izinhlobo zamasampula: igazi, i-serum / i-plasma, izicubu, i-swab, igciwane , njll.
I-TIANamp Virus DNA / RNA Kit (DP315)
● Ukusebenza kahle okuphezulu: Igciwane lekhwalithi ephezulu ye-DNA ne-RNA ingatholwa ngokuhlanzwa okusheshayo ngokuphindeka okuphezulu.
● Ukuhlanzeka okuphezulu: Ukususwa ngokuphelele kwezinto ezingcolisa umoya kanye nezithibisi zokusebenzisa umfula.
● Ukuphepha okuphezulu: Ukukhishwa kwe-reagent organic noma imvula ye-ethanol akudingeki.
Isixazululo Sokuthola i-RNA Virus
1. I-TEasy Automated Pipetting System
● Ukunemba okuphezulu: Ibhlokhi lokupholisa lingagcina izinga lokushisa lesampula elisebenza ngaphansi kwe-7 ℃ ngaphezulu kwamaminithi we-60. I-APM ngayinye ilinganiselwa ngokuya ngamazinga wamazwe omhlaba, anokucaca okuphezulu kune-manual pipetting.
● Ukusebenza okulula: Usayizi omncane. Isisindo esincane. Alikho ithuluzi elidingekayo lokufaka esikhundleni samabhulokhi. Inqubo yokulungiselela eyakhelwe ngaphakathi ye-PCR / qPCR. Ukusebenza okwenziwe ngezandla okwenziwe ngamalungiselelo e-PCR.
● Ukusebenza okubanzi: Kungasetshenziselwa ukufaka amapayipi we-96/384-well plate, i-PCR, i-qPCR, ukutholwa kofuzo nokunye ukuhlolwa okuphezulu.
I-FastKing One Step RT-qPCR Kit (Probe) (FP314)
I-FastKing One Step RT-qPCR Kit (Probe) (FP314) eyenziwe yi-TIANGEN iyithuluzi lokuthwebula elilinganayo le-onestep reverse transcript fluorescence elisuselwa kunqubo yophenyo, eyenzelwe ngokukhethekile ukutholwa kwezakhi zofuzo ezilandelwayo kumasampula ahlukahlukene. I-KingRTase esekhitini iyi-molecular modified reverse transcriptase, enobuhlobo obuqinile be-RNA nokusimama okushisayo, ngokusebenza okuthuthukile kokuphinda kubhalwe phansi kanye nekhono lokwelulwa kwezifanekiso eziyinkimbinkimbi zesakhiwo se-RNA. Isiqalo esisha esishisayo iTaq DNA polymerase nayo iyasetshenziswa ukunikeza ukuphendula kwe-PCR ukusebenza kahle okuphezulu nokukhulisa. Ngaphezu kwalokho, ikhithi yenza izinto zibe lula kakhulu ngokuxuba i-enzyme ye-Taq ne-MLV ibe i-enzyme mix, nokuxuba i-ion buffer, ama-dNTPs, i-PCR stabilizer kanye ne-enhancer ku-MasterMix, ukuze izinyathelo zokuxuba izinto eziningi zibe
lula.
● Ukusebenza kahle okuphezulu: I-reverse transcriptase enhle kakhulu ne-DNA polymerase iqinisekisa ukusebenza kahle kokuphendula okuphezulu
● Ukubuyiselwa emuva okuhle: I-polymerase ingafunda ngesifanekiso se-RNA esinokuqukethwe okuphezulu kwe-GC nesakhiwo esiyinkimbinkimbi sesibili
● Izicelo ezibanzi: Ukusebenza okuphezulu kumathempulethi e-RNA anokungcola okuvela ezinhlotsheni ezahlukahlukene
● Ukuzwela okuphezulu: Amathempulethi aphansi njengo-1 ng angakhonjwa ngokunembile, ikakhulukazi kumathempulethi enqwaba ephansi
Isibonelo sokutholwa kwegciwane le-RNA
I-nucleic acid yomkhuhlane wezinyoni i-H5 ikhishwe yi-TGuide S32 Magnetic Viral DNA / RNA Kit (DP604). I-FastKing One Step RT-qPCR Kit (Probe) (FP314) isetshenziselwe ukutholwa kwe-RT-qPCR kusetshenziswa ama-primer and probes athile we-H5 avian influenza.
I-ABi7500Fast isetshenziselwe ukutholwa kwe-RT-qPCR. Imiphumela ye-H5 avian influenza virus antigen (10-2, 10-3, 10-4, 10-5, 10-6and 10-7 dilution) evela kumasampula angama-200 μl ikhombisa isivuno esikhulu segciwane le-nucleic acid, esingahlangabezana nokulandelayo izidingo zokuloba okuphindayo, i-PCR, i-RT-PCR, i-PCR yesikhathi sangempela, njll. I-PCR yesikhathi sangempela iletha imiphumela ngokuzwela okuphezulu, ukuphindeka okuhle kanye ne-gradient dilution linearity. Ukutholakala okuhlukile kwamasampuli e-viral nucleic acid kungatholakala ngokunembile.
Isikhathi Iposi: Apr-11-2021
