RNA Easy Fast Plant Tissue Kit Featured Image

I-RNA Easy Plant Tissue Kit

Ukuhlanzwa kwe-RNA esezingeni eliphakeme kusuka kuzicubu zezitshalo.

I-RNA Easy Plant Tissue Kit iyikhithi esheshayo yokukhipha i-RNA yezicubu zezitshalo ezakhiwe ngokususelwa kubuchwepheshe bokususa i-genomic DNA obenziwe yi-TIANGEN kuphela. Ama-reagents anobuthi afana ne-β-mercaptoethanol noma i-DTT awadingeki ngesikhathi sokuhlinzwa, futhi yonke inqubo yokukhishwa kwe-RNA ingaqedwa kungakapheli imizuzu engama-30. Ayifanele amasampula amaqabunga ezitshalo ezivamile njengokolweni kanye nommbila, kepha namasampuli acebile e-polysaccharide / polyphenol afana nehlamvu leMalus spectabilis, iqabunga likakotini, iqabunga le-chrysanthemum, i-hibiscus flower, amazambane tuber, ikhabe, ikhukhamba, inalidi yophayini , njll.

Ikati. Cha	Ukupakisha Usayizi
4992880	Ama-preps angama-50

Thumela i-imeyili kithi

Imininingwane yomkhiqizo

Isibonelo sokuhlola

Imibuzo evame ukubuzwa

Amathegi womkhiqizo

Izici

■ Kulula futhi kuyashesha: Ingqikithi ye-RNA ekhishwa kumasampula ezitshalo ingaqedwa kungakapheli imizuzu engama-30.
■ Ukuhambisana okunamandla: Le khithi ayifanele kuphela amasampuli eziqu ezivamile namaqabunga, kodwa futhi namasampuli acebile nge-polysaccharide / polyphenol.
■ Ubuthi obuphephile nobuphansi: Ama-reagents anobuthi afana ne-β-mercaptoethanol, DTT, chloroform, phenol awadingeki.

Izicelo

■ Ilungele ukusebenza ezansi nomfula, kufaka phakathi i-RT-PCR, i-RT-qPCR, i-chip hybridization, i-Northern Blot, i-Dot Blot, ukuhlolwa kwe-PolyA, ukuhumusha kwe-in vitro, ukuhlaziywa kwe-RNase ukuvikelwa nokuhlanganiswa kwamangqamuzana, njll.

Yonke imikhiqizo ingenziwa ngezifiso i-ODM / OEM. Ngemininingwane,sicela uchofoze i-Customized Service (ODM / OEM)

Langaphambilini I-RNA Easy Tissue / Cell Kit

Olandelayo: I-TGuide S32 Magnetic Blood DNA Kit

	Ingqikithi ye-RNA yama-sampuli amaqabunga angama-65 mg (amaqabunga e-clove, amaqabunga e-hibiscus, amaqabunga kakolweni, amaqabunga erayisi, amaqabunga ezithelo ze-ugli, amaqabunga e-Prunus cerasifera, amaqabunga omkhiwane, amaqabunga ansuku zonke, amaqabunga e-Kerria japonica), amasampula we-fungus ayi-150 mg (ama-Lentinus edode) nama-200 amasampula we-mg petal (ama-pet-lily petals) akhishwe kusetshenziswa i-RNA Easy Plant Tissue Kit. Umphumela wokuhlaziya we-Bioanalyzer 2100 we-RNA ephelele kuma-Day-lily petals.
	Umphumela wokuhlaziya we-Agilent Bioanalyzer 2100 we-RNA ephelele evela kumacembe e-Day-lily.
	I-RNA ekhishwe kumasampula ahlukahlukene abhalwe kuthebula kusetshenziswa i-RNA Easy Plant Tissue Kit. Umthamo we-Elution: 50 μl; Ilayisha ivolumu: 2 μl. I-electrophoresis yenziwa ngo-6 V / cm ngemizuzu eyi-15 nge-1% agarose.

Q: Ukuvinjelwa kwekholomu

I-A-1 Cell lysis noma i-homogenization ayanele

- Nciphisa ukusetshenziswa kwesampula, khulisa inani le-lysis buffer, khulisa i-homogenization nesikhathi se-lysis.

Inani elingu-A-2 lesampuli likhulu kakhulu

---- Yehlisa inani lesampula elisetshenzisiwe noma wandise inani le-lysis buffer.

Q: Isivuno se-RNA esiphansi

I-A-1 Inganele i-lysis yeseli noma i-homogenization

- Nciphisa ukusetshenziswa kwesampula, khulisa inani le-lysis buffer, khulisa i-homogenization nesikhathi se-lysis.

Inani elingu-A-2 lesampuli likhulu kakhulu

---- Sicela ubheke amandla wokucubungula aphezulu.

I-A-3 RNA ayikhishwa ngokuphelele kukholamu

---- Ngemuva kokungeza amanzi e-RNase-Free, yiyeke imizuzu embalwa ngaphambi kwe-centrifuging.

I-A-4 Ethanol ku-eluent

---- Ngemuva kokuhlanza, i-centrifuge futhi bese ususa i-buffer yokuwasha ngangokunokwenzeka.

I-A-5 Cell culture medium ayisuswanga ngokuphelele

- Lapho uqoqa amaseli, sicela uqiniseke ukuthi ususa okuphakathi kwesiko ngangokunokwenzeka.

A-6 Amaseli agcinwe ku-RNAstore awasebenzi kahle nge-centrifuged

---- Ukuxinana kwe-RNAstore kukhulu kunesilinganiso esiphakathi sesiko leseli; ngakho-ke amandla e-centrifugal kufanele akhuliswe. Kuphakanyiswa ku-centrifuge ku-3000x g.

Okuqukethwe kwe-A-7 okuphansi kwe-RNA nobuningi kusampula

---- Sebenzisa isampula elihle ukuthola ukuthi isivuno esiphansi sidalwa yisampula.

Q: Ukucekelwa phansi kwe-RNA

A-1 Indaba ayisiyintsha

Izicubu ezintsha kufanele zigcinwe ku-nitrogen ewuketshezi ngokushesha noma ngokushesha zifakwe kwi-reagent ye-RNAstore ukuqinisekisa umphumela wokukhishwa.

Inani elingu-A-2 lesampuli likhulu kakhulu

---- Yehlisa inani lesampula.

I-A-3 RNase contamination

- Yize i-buffer enikezwe kukhithi ingenayo i-RNase, kulula ukungcolisa i-RNase ngesikhathi senqubo yokukhipha futhi kufanele iphathwe ngokunakekela.

Ukungcola kwe-A-4 Electrophoresis

---- Faka esikhundleni se-electrophoresis buffer futhi uqiniseke ukuthi izinto ezisetshenziswayo ne-Loading Buffer azinakho ukungcoliswa yi-RNase.

A-5 Kulayishwa kakhulu i-electrophoresis

---- Yehlisa inani lokulayishwa kwesampula, ukulayishwa komthombo ngamunye akufanele kudlule ku-2 μg.

Umbuzo: Ukungcola kwe-DNA

Inani elingu-1 lesampuli likhulu kakhulu

---- Yehlisa inani lesampula.

A-2 Amanye amasampula anokuqukethwe okuphezulu kwe-DNA futhi angalashwa nge-DNase.

---- Enza ukwelashwa kwe-RNase-Free DNase kusixazululo esitholakele se-RNA, futhi i-RNA ingasetshenziswa ngqo ekuhlolweni okulandelayo ngemuva kokwelashwa, noma ingaqhubeka ihlanzwe ngamakhithi wokuhlanzwa we-RNA.

Q: Ungayisusa kanjani i-RNase kokudliwayo kokuhlola kanye nama-glasswares?

Okwezingilazi, kubhakwe ku-150 ° C ngehora elingu-4. Kwiziqukathi zepulasitiki, ucwiliswe ku-0.5 M NaOH ngemizuzu eyi-10, bese ugeza kahle ngamanzi angenawo i-RNase bese uvala inzalo ukususa i-RNase ngokuphelele. Ama-reagents noma izixazululo ezisetshenziswe ekuhlolweni, ikakhulukazi amanzi, kumele zingabi ne-RNase. Sebenzisa amanzi angenawo ama-RNase kuwo wonke amalungiselelo e-reagent (engeza amanzi ebhodleleni lengilazi elihlanzekile, engeza i-DEPC ekugxileni kokugcina okungu-0.1% (V / V), unyakazise ubusuku kanye ne-autoclave).

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