■ Ilungele igazi elisha lonke lezinhlobo ezahlukahlukene, okulula ukusebenziseka.
■ Ifakwe i-RNase-Free Filtration Column CS ukususa ngempumelelo ukungcola.
■ I-buffer eyakhiwe ngokukhethekile ingaqinisekisa ukukhishwa kwe-RNA okusebenzayo futhi okuzinzile kokuhlolwa okwehla nokwehla.
■ Ukusebenza okuphephile nokwethembekile, akudingeki ukukhishwa kwe-phenol / chloroform.
I-RT-PCR, i-Northern Blot, i-RT-qPCR, ukuhlaziywa kwe-chip, ukulandelana okuphezulu, ukuhlolwa kwe-PolyA, ukuhlaziywa kokuvikelwa kwe-RNase, ukuhumusha kwe-vitro, njll.
Yonke imikhiqizo ingenziwa ngezifiso i-ODM / OEM. Ngemininingwane,sicela uchofoze i-Customized Service (ODM / OEM)
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Umdwebo 1. I-RNA ihlanzwe kusuka ku-100 μl yegazi elisha lamagundane kuma-anticoagulants ahlukahlukene kusetshenziswa i-RNAprep Pure Hi-Blood Kit. I-4-6 μl yama-50 μl eluates alayishwe kulayini ngamunye. M: I-TIANGEN DNA Umaka III. |
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Umdwebo 2. I-RNA ihlanzwe kusuka ku-100 μl yegazi legundane elisha kusetshenziswa i-RNAprep Pure Hi-Blood Kit. I-4-6 μl yama-50 μl eluates alayishwe kulayini ngamunye. M: I-TIANGEN DNA Umaka III. |
I-A-1 Cell lysis noma i-homogenization ayanele
- Nciphisa ukusetshenziswa kwesampula, khulisa inani le-lysis buffer, khulisa i-homogenization nesikhathi se-lysis.
Inani elingu-A-2 lesampuli likhulu kakhulu
---- Yehlisa inani lesampula elisetshenzisiwe noma wandise inani le-lysis buffer.
I-A-1 Inganele i-lysis yeseli noma i-homogenization
- Nciphisa ukusetshenziswa kwesampula, khulisa inani le-lysis buffer, khulisa i-homogenization nesikhathi se-lysis.
Inani elingu-A-2 lesampuli likhulu kakhulu
---- Sicela ubheke amandla wokucubungula aphezulu.
I-A-3 RNA ayikhishwa ngokuphelele kukholamu
---- Ngemuva kokungeza amanzi e-RNase-Free, yiyeke imizuzu embalwa ngaphambi kwe-centrifuging.
I-A-4 Ethanol ku-eluent
---- Ngemuva kokuhlanza, i-centrifuge futhi bese ususa i-buffer yokuwasha ngangokunokwenzeka.
I-A-5 Cell culture medium ayisuswanga ngokuphelele
- Lapho uqoqa amaseli, sicela uqiniseke ukuthi ususa okuphakathi kwesiko ngangokunokwenzeka.
A-6 Amaseli agcinwe ku-RNAstore awasebenzi kahle nge-centrifuged
---- Ukuxinana kwe-RNAstore kukhulu kunesilinganiso esiphakathi sesiko leseli; ngakho-ke amandla e-centrifugal kufanele akhuliswe. Kuphakanyiswa ku-centrifuge ku-3000x g.
Okuqukethwe kwe-A-7 okuphansi kwe-RNA nobuningi kusampula
---- Sebenzisa isampula elihle ukuthola ukuthi isivuno esiphansi sidalwa yisampula.
A-1 Indaba ayisiyintsha
Izicubu ezintsha kufanele zigcinwe ku-nitrogen ewuketshezi ngokushesha noma ngokushesha zifakwe kwi-reagent ye-RNAstore ukuqinisekisa umphumela wokukhishwa.
Inani elingu-A-2 lesampuli likhulu kakhulu
---- Yehlisa inani lesampula.
I-A-3 RNase contamination
- Yize i-buffer enikezwe kukhithi ingenayo i-RNase, kulula ukungcolisa i-RNase ngesikhathi senqubo yokukhipha futhi kufanele iphathwe ngokunakekela.
Ukungcola kwe-A-4 Electrophoresis
---- Faka esikhundleni se-electrophoresis buffer futhi uqiniseke ukuthi izinto ezisetshenziswayo ne-Loading Buffer azinakho ukungcoliswa yi-RNase.
A-5 Kulayishwa kakhulu i-electrophoresis
---- Yehlisa inani lokulayishwa kwesampula, ukulayishwa komthombo ngamunye akufanele kudlule ku-2 μg.
Inani elingu-1 lesampuli likhulu kakhulu
---- Yehlisa inani lesampula.
A-2 Amanye amasampula anokuqukethwe okuphezulu kwe-DNA futhi angalashwa nge-DNase.
---- Enza ukwelashwa kwe-RNase-Free DNase kusixazululo esitholakele se-RNA, futhi i-RNA ingasetshenziswa ngqo ekuhlolweni okulandelayo ngemuva kokwelashwa, noma ingaqhubeka ihlanzwe ngamakhithi wokuhlanzwa we-RNA.
Okwezingilazi, kubhakwe ku-150 ° C ngehora elingu-4. Kwiziqukathi zepulasitiki, ucwiliswe ku-0.5 M NaOH ngemizuzu eyi-10, bese ugeza kahle ngamanzi angenawo i-RNase bese uvala inzalo ukususa i-RNase ngokuphelele. Ama-reagents noma izixazululo ezisetshenziswe ekuhlolweni, ikakhulukazi amanzi, kumele zingabi ne-RNase. Sebenzisa amanzi angenawo ama-RNase kuwo wonke amalungiselelo e-reagent (engeza amanzi ebhodleleni lengilazi elihlanzekile, engeza i-DEPC ekugxileni kokugcina okungu-0.1% (V / V), unyakazise ubusuku kanye ne-autoclave).
Kusukela yasungulwa, imboni yethu ibilokhu ithuthukisa imikhiqizo esezingeni lomhlaba yokuqala ngokunamathela esimisweni
wekhwalithi kuqala. Imikhiqizo yethu izuze idumela elihle kakhulu embonini futhi ithembekile phakathi kwamakhasimende amasha namadala ..