I-RNAprep Pure Hi-Blood Kit

Ukuhlanzwa kwe-RNA esezingeni eliphezulu futhi ezinzile egazini.

I-RNAprep Pure Hi-Blood Kit ikhipha kahle i-RNA ephelele egazini eliphelele kanye negazi elinama-anticoagulants amaningi avela ezinhlotsheni ezahlukahlukene. Okokusebenza kwe-silicon matrix okusetshenziswe kukholamu ye-adsorption kuyinto entsha eyingqayizivele eyenziwe yi-TIANGEN, ekhangisa kahle futhi ngokukhethekile i-RNA, futhi isuse amaprotheni okungcola ngezinga elikhulu kakhulu. I-RNA ekhishiwe ingasetshenziswa kuzilingo ezahlukahlukene ezisezansi ezifana ne-RT-PCR, i-RT-qPCR, ukuhlaziywa kwe-chip, ukulandelana okuphezulu, i-Northern Blot, i-Dot Blot, ukuhlolwa kwe-PolyA, ukuhumusha kwe-vitro, ukuhlaziywa kwe-RNase yokuvikela, ukuhlanganiswa kwamangqamuzana, njll.

Ikati. Cha	Ukupakisha Usayizi
4992903	Ama-preps angama-50

Thumela i-imeyili kithi

Imininingwane yomkhiqizo

Isibonelo sokuhlola

Imibuzo evame ukubuzwa

Amathegi womkhiqizo

Izici

■ Ilungele igazi elisha lonke lezinhlobo ezahlukahlukene, okulula ukusebenziseka.
■ Ifakwe i-RNase-Free Filtration Column CS ukususa ngempumelelo ukungcola.
■ I-buffer eyakhiwe ngokukhethekile ingaqinisekisa ukukhishwa kwe-RNA okusebenzayo futhi okuzinzile kokuhlolwa okwehla nokwehla.
■ Ukusebenza okuphephile nokwethembekile, akudingeki ukukhishwa kwe-phenol / chloroform.

Izicelo

I-RT-PCR, i-Northern Blot, i-RT-qPCR, ukuhlaziywa kwe-chip, ukulandelana okuphezulu, ukuhlolwa kwe-PolyA, ukuhlaziywa kokuvikelwa kwe-RNase, ukuhumusha kwe-vitro, njll.

Yonke imikhiqizo ingenziwa ngezifiso i-ODM / OEM. Ngemininingwane,sicela uchofoze i-Customized Service (ODM / OEM)

Langaphambilini Ikhithi ye-RNAprep Pure Plant Plus

Olandelayo: I-RNA Easy Tissue / Cell Kit

	Umdwebo 1. I-RNA ihlanzwe kusuka ku-100 μl yegazi elisha lamagundane kuma-anticoagulants ahlukahlukene kusetshenziswa i-RNAprep Pure Hi-Blood Kit. I-4-6 μl yama-50 μl eluates alayishwe kulayini ngamunye. M: I-TIANGEN DNA Umaka III.
	Umdwebo 2. I-RNA ihlanzwe kusuka ku-100 μl yegazi legundane elisha kusetshenziswa i-RNAprep Pure Hi-Blood Kit. I-4-6 μl yama-50 μl eluates alayishwe kulayini ngamunye. M: I-TIANGEN DNA Umaka III.

Q: Ukuvinjelwa kwekholomu

I-A-1 Cell lysis noma i-homogenization ayanele

- Nciphisa ukusetshenziswa kwesampula, khulisa inani le-lysis buffer, khulisa i-homogenization nesikhathi se-lysis.

Inani elingu-A-2 lesampuli likhulu kakhulu

---- Yehlisa inani lesampula elisetshenzisiwe noma wandise inani le-lysis buffer.

Q: Isivuno se-RNA esiphansi

I-A-1 Inganele i-lysis yeseli noma i-homogenization

- Nciphisa ukusetshenziswa kwesampula, khulisa inani le-lysis buffer, khulisa i-homogenization nesikhathi se-lysis.

Inani elingu-A-2 lesampuli likhulu kakhulu

---- Sicela ubheke amandla wokucubungula aphezulu.

I-A-3 RNA ayikhishwa ngokuphelele kukholamu

---- Ngemuva kokungeza amanzi e-RNase-Free, yiyeke imizuzu embalwa ngaphambi kwe-centrifuging.

I-A-4 Ethanol ku-eluent

---- Ngemuva kokuhlanza, i-centrifuge futhi bese ususa i-buffer yokuwasha ngangokunokwenzeka.

I-A-5 Cell culture medium ayisuswanga ngokuphelele

- Lapho uqoqa amaseli, sicela uqiniseke ukuthi ususa okuphakathi kwesiko ngangokunokwenzeka.

A-6 Amaseli agcinwe ku-RNAstore awasebenzi kahle nge-centrifuged

---- Ukuxinana kwe-RNAstore kukhulu kunesilinganiso esiphakathi sesiko leseli; ngakho-ke amandla e-centrifugal kufanele akhuliswe. Kuphakanyiswa ku-centrifuge ku-3000x g.

Okuqukethwe kwe-A-7 okuphansi kwe-RNA nobuningi kusampula

---- Sebenzisa isampula elihle ukuthola ukuthi isivuno esiphansi sidalwa yisampula.

Q: Ukucekelwa phansi kwe-RNA

A-1 Indaba ayisiyintsha

Izicubu ezintsha kufanele zigcinwe ku-nitrogen ewuketshezi ngokushesha noma ngokushesha zifakwe kwi-reagent ye-RNAstore ukuqinisekisa umphumela wokukhishwa.

Inani elingu-A-2 lesampuli likhulu kakhulu

---- Yehlisa inani lesampula.

I-A-3 RNase contamination

- Yize i-buffer enikezwe kukhithi ingenayo i-RNase, kulula ukungcolisa i-RNase ngesikhathi senqubo yokukhipha futhi kufanele iphathwe ngokunakekela.

Ukungcola kwe-A-4 Electrophoresis

---- Faka esikhundleni se-electrophoresis buffer futhi uqiniseke ukuthi izinto ezisetshenziswayo ne-Loading Buffer azinakho ukungcoliswa yi-RNase.

A-5 Kulayishwa kakhulu i-electrophoresis

---- Yehlisa inani lokulayishwa kwesampula, ukulayishwa komthombo ngamunye akufanele kudlule ku-2 μg.

Umbuzo: Ukungcola kwe-DNA

Inani elingu-1 lesampuli likhulu kakhulu

---- Yehlisa inani lesampula.

A-2 Amanye amasampula anokuqukethwe okuphezulu kwe-DNA futhi angalashwa nge-DNase.

---- Enza ukwelashwa kwe-RNase-Free DNase kusixazululo esitholakele se-RNA, futhi i-RNA ingasetshenziswa ngqo ekuhlolweni okulandelayo ngemuva kokwelashwa, noma ingaqhubeka ihlanzwe ngamakhithi wokuhlanzwa we-RNA.

Q: Ungayisusa kanjani i-RNase kokudliwayo kokuhlola kanye nama-glasswares?

Okwezingilazi, kubhakwe ku-150 ° C ngehora elingu-4. Kwiziqukathi zepulasitiki, ucwiliswe ku-0.5 M NaOH ngemizuzu eyi-10, bese ugeza kahle ngamanzi angenawo i-RNase bese uvala inzalo ukususa i-RNase ngokuphelele. Ama-reagents noma izixazululo ezisetshenziswe ekuhlolweni, ikakhulukazi amanzi, kumele zingabi ne-RNase. Sebenzisa amanzi angenawo ama-RNase kuwo wonke amalungiselelo e-reagent (engeza amanzi ebhodleleni lengilazi elihlanzekile, engeza i-DEPC ekugxileni kokugcina okungu-0.1% (V / V), unyakazise ubusuku kanye ne-autoclave).

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