SuperReal PreMix Color (SYBR Green) Featured Image

Umbala weSuperReal PreMix (SYBR Green)

Ukucabangela kanye nedayi ethize ye-reagent yokulinganisa.

Umkhiqizo uyi-reagent ekhethekile ye-PCR yesikhathi sangempela usebenzisa i-SYBR Green I chimeric fluorescence. Umkhiqizo uyi-2 × okuhlushwa i-PreMix exutshwe nokuhlungwa okukhulu kwe-SYBR Green engiyidinga ekuphenduleni kwe-PCR yangempela. Inkomba ingeziwe futhi kumkhiqizo, enenzuzo ekulayisheni inani elikhulu lamasampula futhi inciphisa amathuba okungaxhunyaniseki.

ISuperReal Colour PreMix isebenzisa into eyingqayizivele eyisiqalo esishisayo ye-DNA polymerase (i-HotStart eguqulwe ngamakhemikhali Isi-Taq I-DNA polymerase ne-anti-anti eguqulwe i-Anti Isi-Taq I-DNA polymerase), futhi ibonakala ngokusebenza kahle kokukhulisa okuphezulu, ukucaciswa okuphezulu kokukhulisa nobubanzi obunokwethenjelwa obuhlanganiswe nenqubo ye-buffer elungiselelwe ngokucophelela. I-reagent ethuthukisiwe inezinzuzo ezivelele ekucacisweni kokukhulisa ngemuva kokulungiswa kwengxenye.

Ikati. Cha	Ukupakisha Usayizi
4992777	20 ×l × 125 rxn
4992876	20 ×l × 500 rxn
4992902	20 ×l × 5000 rxn

Thumela i-imeyili kithi

Imininingwane yomkhiqizo

Isibonelo sokuhlola

Amathegi womkhiqizo

Izici

■ Izinkomba zombala ziyangezwa kuma-reagents ukugwema amaphutha wokungezwa kwesampula noma ukweqiwa ngenxa yenani elikhulu lamasampuli.
■ Inani le-fluorescence ephezulu, ukuzwela okuphezulu kanye nokucaciswa okuphezulu.
■ Ngemuva kokutholwa okuqinile, inkomba ngeke iphazamise inani le-fluorescence nemiphumela yokubala kukadayi.
■ Kufanele izinhlobonhlobo zezinsimbi ezijwayelekile zesikhathi sangempela se-PCR (iRoche, i-Bio-rad, i-ABI, njll.).

SuperReal PreMix Color (SYBR Green）

Izicelo

Ifanele izinhlobo ezahlukahlukene zokuhlolwa njengokuhlaziywa kwenkulumo nokutholwa kwe-nucleic acid ngendlela ye-SYBR Green kuzinsimbi ezahlukahlukene zesikhathi sangempela se-PCR. Ilungele ikakhulukazi ukuhlolwa okuphezulu okuphumayo ngenani elikhulu lamasampula nokusebenza kokulayishwa okuyisampula okuyinkimbinkimbi.

Yonke imikhiqizo ingenziwa ngezifiso i-ODM / OEM. Ngemininingwane,sicela uchofoze i-Customized Service (ODM / OEM)

Langaphambilini Ngokwe-Genotyping qPCR PreMix (Probe)

Olandelayo: Umbala weSuperReal PreMix (Probe)

Umdwebo. 1. Amagrafu ama-amplifi cation curves namajika ancibilikayo atholakalayo ngokulandelana kokulinganisa uhlobo lomuntu lwe-HS1 kusetshenziswa i-TIANGEN SuperReal PreMix Plus (SYBR Green) (A) ne-SuperReal PreMix Colour (SYBR Green) (B). Isifanekiso sihlanjululwe ama-gradients wokuhlushwa ama-5. Amanani we-Ct (C) atholwe ngokuqhathanisa akhombisa ukuthi ukukhuliswa kwamakhithi amabili kunokuzwela okukhulu, ukucaciswa nokuzinza okukhulu. Ukuhlolwa kwenziwa ku-Roche LightCycler480.

Umdwebo 2. Amagrafu wejika lokukhulisa nokujika okuncibilikayo okutholwe ngokulinganisa isilinganiso sofuzo lwe-HS5 ngokulandelana kusetshenziswa i-TIANGEN SuperReal PreMix Colour (SYBR Green) nomkhiqizo ofanele ovela ku-Supplier T (A: TIANGEN, B: Supplier T. Hlanganisa isifanekiso sama-gradients wokuhlushwa ama-5, bese uqhathanisa amanani we-Ct (C). Umphumela ukhombisa ukuthi i-SuperReal PreMix Colour (SYBR Green) inokuzwela okuphezulu, ukucaciswa nokuzinza. Ukuhlolwa kwenziwa ku-Bio-Rad CFX96.

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