■ Kulula ukuyisebenzisa: Ukusebenza kwesoftware kungenziwa kalula ngaphakathi kwe-1 h. Uhlelo lokulungiselela lwe-PCR / qPCR olwakhelwe ngaphakathi lungashintshwa futhi ludluliswe ngokushesha.
■ Okusebenzisekayo okuhambisanayo: Ithiphu ye-pipette eshintshashintshayo nohlelo lwe-Beckman Biomek 3000.
■ Ukugcinwa okulula: Imodyuli ezenzakalelayo yokudonsa amapayipi (i-APM) ingashintshwa futhi ibuyiselwe emuva ukulungisa iphutha.
■ Ukunemba okuphezulu nokunemba.
Yonke imikhiqizo ingenziwa ngezifiso i-ODM / OEM. Ngemininingwane,sicela uchofoze i-Customized Service (ODM / OEM)
Umdwebo 1: Imiphumela ejwayelekile yejika le-qPCR ikhombisa ukuphindeka okuhle Amasampula e-cDNA wamaseli we-7 μl NIH 3T3 ahlanjululwa amahlandla ama-4 nge-21 μl yamanzi ngesilinganiso se-1: 4. Ithuluzi le-PCR le-Roche LightCycler 480 langempela le-fluorescence kanye ne-SuperReal PreMix Plus (i-SYBR Green) isetshenziselwe ukutholwa. |
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Umdwebo 2: Ukunemba okuphezulu kuqhathaniswa nokupaywa ngesandla (Kwesobunxele: Imanuwali; Kwesokudla: I-TEasy Automated Pipetting) Ukuphindwa okungu-4 kokukhuliswa komuntu kwe-GAPDH (ijika eliphezulu). Faka i-2 μl cDNA kuye ku-18 μl MasterMix ukuze wakhe uhlelo lokuphendula olungama-20 μl. I-Roche LightCycler 480 real-time fluorescence quantitative PCR instrument kanye ne-SuperReal PreMix Plus (SYBR Green) isetshenziselwe ukutholwa. |
Kusukela yasungulwa, imboni yethu ibilokhu ithuthukisa imikhiqizo esezingeni lomhlaba yokuqala ngokunamathela esimisweni
wekhwalithi kuqala. Imikhiqizo yethu izuze idumela elihle kakhulu embonini futhi ithembekile phakathi kwamakhasimende amasha namadala ..