I-TGuide S32 Magnetic Plant DNA Kit

Ikhithi isebenzisa ubuhlalu bukazibuthe obunomsebenzi owehlukile wokuhlukanisa nohlelo oluyingqayizivele lokuhlukanisa nokuhlanza i-DNA esezingeni eliphezulu kwizicubu zezitshalo ezahlukahlukene. Ubuhlalu obuyingqayizivele obushunyekiwe bunamathele kakhulu kwi-nucleic acid ngaphansi kwezimo ezithile, futhi lapho izimo zishintsha, ubuhlalu bamagnetic buzokhipha i-adsorbed nucleic acid, ngaleyo ndlela kufezekiswe injongo yokwehlukaniswa okusheshayo nokuhlanzwa kwe-nucleic acid.
Umkhiqizo uhambelana ngokuphelele ne-TGuide S32 Automated Nucleic Acid Extractor, ngaleyo ndlela kuqaphele ukudluliswa kobuhlalu bamagnetic nama-nucleic acid. Yonke inqubo yokuhlola iphephile futhi ilula. Izingcezu ze-genomic ze-genomic ezikhishwe zikhulu ngosayizi, zinobumsulwa obuphakeme nekhwalithi ezinzile nethembekile.
I-DNA ehlanzwe yikhithi ilungele imisebenzi eyahlukahlukene ejwayelekile, kufaka phakathi ukugaywa kwe-enzyme, i-PCR, ukwakhiwa kwelabhulali, i-Southern blot nezinye izivivinyo.

Ikati. Cha Ukupakisha Usayizi
4992989 96 preps

Imininingwane yomkhiqizo

Imibuzo evame ukubuzwa

Amathegi womkhiqizo

Izici

■ Kulula futhi kuyashesha: Ultrapure genomic DNA ingatholakala kungakapheli ihora elilodwa.
■ Ukusebenza okubanzi: Kufanele izicubu zezitshalo ezahlukahlukene, ikakhulukazi izitshalo ezicebile ze-polysaccharide / polyphenol.
■ Kuphephile futhi akunabo ubuthi: Azikho izidalwa ezinobuthi ezinobuthi ezifana ne-phenol / chloroform ezidingekayo.
■ Ukuhlanzeka okuphezulu: I-DNA etholakele inokuhlanzeka okuphezulu futhi ingasetshenziswa ngqo ekutholeni i-chip, ukulandelana okuphezulu kokunye okunye ukuhlolwa.

Yonke imikhiqizo ingenziwa ngezifiso i-ODM / OEM. Ngemininingwane,sicela uchofoze i-Customized Service (ODM / OEM)


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  • Olandelayo:

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    Q: I-DNA encane noma ayikho ku-eluent.

    A-1 Ukuhlushwa okuphansi kwamaseli noma igciwane kusampula yokuqala -Nothisa ukuhlushwa kwamaseli noma amagciwane.

    I-A-2 I-lysis enganele yamasampuli-Amasampula awakaxutshwa kahle ne-lysis buffer. Kuphakanyiswa ukuthi uhlanganise kahle nge-pulse-vortexing izikhathi eziyi-1-2. —I-lysis enganele yeseli ebangelwe ukwehla komsebenzi we-proteinase K. —Ukungalingani kwe-cell lysis noma ukwehla kwamaprotheni ngenxa yesikhathi esanele sokugeza. Kuphakanyiswa ukusika izicubu zibe yizicucu ezincane bese unweba isikhathi sokugeza ukuze ususe yonke insalela ku-lysate.

    I-A-3 Ukukhangisa okwanele kwe-DNA. -Ayikho i-ethanol noma amaphesenti aphansi esikhundleni se-100% ethanol engezwe ngaphambi kokuthi i-lysate idluliselwe kukholamu ye-spin.

    A-4 Inani le-pH le-elution buffer liphansi kakhulu. - Lungisa i-pH iye phakathi kuka-8.0-8.3.

    Q: I-DNA ayisebenzi kahle ekuhlolweni kokuphendula kwe-enzymatic reaction.

    I-ethanol eyinsalela ku-eluent.

    —Kunensalela yokuwasha i-PW esikhaleni. I-ethanol ingasuswa ngokufaka phakathi ikholamu ye-spin ngemizuzu engu-3-5, bese ifakwa ekamelweni lokushisa noma ku-50 ℃ incubator ye-1-2 min.

    Q: Ukucekelwa phansi kwe-DNA

    A-1 Isampula akuyona entsha. —Khipha i-DNA eyisampula enhle njengokulawula ukunquma ukuthi ngabe i-DNA esampulweni yehle yini.

    Ukwelashwa kwangaphambili okungalungile. —Kudalwa ukugaya okweqile kwe-nitrogen, uketshezi olubuyisa umswakama, noma inani elikhulu kakhulu lesampula.

    Q: Ungakwenza kanjani ukwelashwa kwangaphambili kwe-gDNA?

    Ama-pretreatment kufanele ahluke kumasampula ahlukile. Ngamasampula ezitshalo, qiniseka ukuthi ugaya kahle i-nitrogen engamanzi. Ngamasampuli ezilwane, homogenate noma gaya kahle i-nitrogen engamanzi. Kumasampuli anezindonga zamaseli okunzima ukugqashuka, njengama-bacterium e-G + nemvubelo, kuphakanyiswa ukusebenzisa i-lysozyme, i-lyticase noma izindlela zemishini yokwephula izindonga zeseli.

    Q: Uyini umehluko phakathi kwamakhithi amathathu wokukhipha we-gDNA 4992201/4992202, 4992724/4992725, 4992709/4992710?

    I-4992201/4992202 iPlant Genomic DNA Kit isebenzisa indlela esekwe kukholamu edinga i-chloroform yokukhipha. Ilungele ikakhulukazi amasampula ezitshalo ezahlukahlukene, kanye ne-powder eyomile yesitshalo. IHi-DNAsecure Plant Kit nayo isekelwe kwikholomu, kepha asikho isidingo sokukhishwa kwe-phenol / chloroform, okuyenza iphephe futhi ingabi nobuthi. Ifanele izitshalo ezinama-polysaccharides aphezulu nokuqukethwe kwe-polyphenol. I-4992709/4992710 DNAquick Plant System isebenzisa indlela esekelwa uketshezi. Ukukhishwa kwePhenol / chloroform akudingeki futhi. Inqubo yokuhlanzwa ilula futhi iyashesha ngaphandle komkhawulo wamanani okuqala esampula, ngakho-ke abasebenzisi bangalungisa inani ngokuvumelana nezidingo zokuhlola. Usayizi omkhulu wezingcezu ze-gDNA ungatholakala ngesivuno esikhulu.

    Yini isivuno esilinganiselwayo se-gDNA kusuka kusampula yegazi engu-1 ml nge-TIANamp Blood DNA Kit?

    I-genomic DNA ikhishwe emananini ahlukene amasampuli egazi lomuntu yi-TIANamp Blood DNA Kit. Imiphumela imi kanje. Imiphumela ibalwe njengereferensi kuphela, imiphumela yangempela yokukhipha incike ezimweni zamasampuli.

    faq

    Q: Ingabe i-4992207/4992208 ne-4992722/4992723 ingasetshenziswa ukukhipha ama-blood clots DNA?

    Ukukhishwa kwegazi kwe-DNA kwegazi kungenziwa kusetshenziswa ama-reagents ahlinzekwe kulawa makhithi amabili ngokumane uguqule umthetho olandelwayo uye emyalweni othize wokukhishwa kwegazi kwe-DNA. Ikhophi ethambile ye-clot yegazi ye-DNA protocol ingakhishwa uma ucela.

    Q: Lapho usebenzisa i-TIANamp Genomic DNA Kit, ungaziqhekeza kanjani izicubu ezintsha zibe ukumiswa kweseli?

    Misa okwesikhashana isampula elisha nge-1 ml PBS, usawoti ojwayelekile noma i-TE buffer. Homogenize Ngokuphelele isampula nge-homogenizer bese uqoqa imvula phansi kwephubhu nge-centrifuging. Lahla amandla angaphezu kwawemvelo, uphinde uvuselele ukugqashuka nge-200 μl buffer GA. Ukuhlanzwa kwe-DNA okulandelayo kungenziwa ngokuya ngemiyalo.

    Q: Ungawukhetha kanjani umkhiqizo wokukhishwa kwe-DNA kusuka kumasampula e-plasma, serum kanye ne-fluid fluid?

    Ukuhlanzwa kwe-gDNA kumasampula e-plasma, serum kanye ne-fluid fluid, Kunconywa i-TIANamp Micro DNA Kit. Ukuhlanzwa kwegciwane i-gDNA kusuka kumasampula we-serum / plasma, Kunconywa i-TIANamp Virus DNA / RNA Kit. Ukuhlanzwa kwe-gDNA yebhaktheriya kusuka kumasampula we-serum ne-plasma, i-TIANamp Bacteria DNA Kit inconywa (i-lysozyme kufanele ifakwe ibhaktheriya elihle). Kumasampula amathe, i-Hi-Swab DNA Kit ne-TIANamp Bacteria DNA Kit kuyanconywa.

    Q: Ungawakhetha kanjani amakhithi okukhishwa kwe-gDNA kusuka kumasampula esikhunta?

    I-DNAsecure Plant Kit noma i-DNAquick Plant System inconyelwa ukukhishwa kwefungal genome. Ngokukhishwa kwemvubelo ye-genome, Kunconywa i-TIANamp Yeast DNA Kit (i-lyticase kufanele izilungiselele).

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