I-TIANamp FFPE DNA Kit

Ukuhlanzwa okuphezulu kwe-DNA kusuka kwizicubu ezifakwe ku-formalin, okugxilwe kuphalafini ngokwelashwa kwe-xylene.

I-TIANamp FFPE DNA Kit yenzelwe ukuhlanzwa kwe-DNA kusuka ezingxenyeni zezicubu ze-FFPE. Isebenzisa i-xylene ukususa uphalafini, futhi inikezela ngezimo ezihlukile ze-lysis ze-DNA ezokhishwa kucezu lwethishu. Ihlanganiswe nolwelwesi olususelwa ku-silica olususelwa ekukhetheni nesistimu ye-elution eguquguqukayo, le kit ingakhulisa i-DNA esezingeni eliphakeme ibe yivolumu encane. I-DNA ehlanzekile ingasebenza ngqo njengezifanekiso zokutholwa kwangaphansi noma ezinye izinhlelo zokusebenza.

Ikati. Cha	Ukupakisha Usayizi
4992524	Ama-preps angama-50

Thumela i-imeyili kithi

Imininingwane yomkhiqizo

Isibonelo sokuhlola

Imibuzo evame ukubuzwa

Amathegi womkhiqizo

Izici

■ Ukuhlanzeka okuphezulu: Ulwelwesi lwe-Silica lususa ukungcola okuningi.
■ Ukusetshenziswa okubanzi: Ilungele isigaba sikapharafini, ibhulokhi kapharafini, namasampuli ashumekwe ngo-formalin.
■ Ukusebenza ngokushesha: I-DNA ingatholakala kungakapheli ihora elilodwa.

Ukucaciswa

Thayipha: Spin ikholomu isuselwe
Isampula: Isigaba sikapharafini, ibhulokhi kapharafini, namasampuli ashumekiwe e-formalin
Target: I-Genomic DNA
Ivolumu yokuqala: izingcezu ezi-5-8 zezingxenye zikapharafini noma izicubu ezingama-30 mg kupharafini noma e-formalin
Isikhathi sokusebenza: ~ 1 ihora
Izicelo ezisezansi: Ukuhlaziywa kwe-PCR, SNP genotype, ukuhlaziywa kwe-STR, ukuhlaziywa kwe-pharmacogenomics, ukwakhiwa kwelabhulali ye-NGS, njll.

Yonke imikhiqizo ingenziwa ngezifiso i-ODM / OEM. Ngemininingwane,sicela uchofoze i-Customized Service (ODM / OEM)

Langaphambilini I-TIANquick FFPE DNA Kit

Olandelayo: I-TIANprep N96 Plasmid Kit

I-genomic DNA ekhishwe yi-TIANamp FFPE DNA Kit kanye nomkhiqizo ofanele ovela kuMhlinzeki A wakhuliselwa ku-190 bp, 330 bp, 500 bp, 650 bp no-1300 bp. Umphumela ukhombisa ukuthi i-DNA ekhishwe yi-TIANamp FFPE DNA Kit inokuvunwa okuphezulu, ubumsulwa obuphakeme ikakhulukazi izingcezu ezinkulu.

Izinto zokuqala: Amasampula wesibindi se-FFPE sesibindi (izingcezu ezi-5, 10 μm / ucezu)
Inqubo: Landela imiyalelo yomkhiqizo ngamunye.
Ukulayishwa kwe-Gel: i-gDNA yaklonyeliswa nge-50 μl elution buffer futhi yandiswa yaba yi-190 bp, 330 bp 500 bp, 650 bp ne-1300 bp. I-5 μl yemikhiqizo engama-20 μl ye-PCR yalayishwa kulayini ngamunye ku-1% ijeli ye-agarose. I-electrophoresis yenziwa ngo-6 V / cm ngemizuzu engama-20.
M: Umaka we-TIANGEN D2000;
T: I-TIANamp FFPE DNA Kit;
A: Umkhiqizo ofanele ovela kuMhlinzeki A.

Q: I-DNA encane noma ayikho ku-eluent.

A-1 Ukuhlushwa okuphansi kwamaseli noma igciwane kusampula yokuqala -Nothisa ukuhlushwa kwamaseli noma amagciwane.

I-A-2 I-lysis enganele yamasampuli-Amasampula awakaxutshwa kahle ne-lysis buffer. Kuphakanyiswa ukuthi uhlanganise kahle nge-pulse-vortexing izikhathi eziyi-1-2. —I-lysis enganele yeseli ebangelwe ukwehla komsebenzi we-proteinase K. —Ukungalingani kwe-cell lysis noma ukwehla kwamaprotheni ngenxa yesikhathi esanele sokugeza. Kuphakanyiswa ukusika izicubu zibe yizicucu ezincane bese unweba isikhathi sokugeza ukuze ususe yonke insalela ku-lysate.

I-A-3 Ukukhangisa okwanele kwe-DNA. -Ayikho i-ethanol noma amaphesenti aphansi esikhundleni se-100% ethanol engezwe ngaphambi kokuthi i-lysate idluliselwe kukholamu ye-spin.

A-4 Inani le-pH le-elution buffer liphansi kakhulu. - Lungisa i-pH iye phakathi kuka-8.0-8.3.

Q: I-DNA ayisebenzi kahle ekuhlolweni kokuphendula kwe-enzymatic reaction.

I-ethanol eyinsalela ku-eluent.

—Kunensalela yokuwasha i-PW esikhaleni. I-ethanol ingasuswa ngokufaka phakathi ikholamu ye-spin ngemizuzu engu-3-5, bese ifakwa ekamelweni lokushisa noma ku-50 ℃ incubator ye-1-2 min.

Q: Ukucekelwa phansi kwe-DNA

A-1 Isampula akuyona entsha. —Khipha i-DNA eyisampula enhle njengokulawula ukunquma ukuthi ngabe i-DNA esampulweni yehle yini.

Ukwelashwa kwangaphambili okungalungile. —Kudalwa ukugaya okweqile kwe-nitrogen, uketshezi olubuyisa umswakama, noma inani elikhulu kakhulu lesampula.

Q: Ungakwenza kanjani ukwelashwa kwangaphambili kwe-gDNA?

Ama-pretreatment kufanele ahluke kumasampula ahlukile. Ngamasampula ezitshalo, qiniseka ukuthi ugaya kahle i-nitrogen engamanzi. Ngamasampuli ezilwane, homogenate noma gaya kahle i-nitrogen engamanzi. Kumasampuli anezindonga zamaseli okunzima ukugqashuka, njengama-bacterium e-G + nemvubelo, kuphakanyiswa ukusebenzisa i-lysozyme, i-lyticase noma izindlela zemishini yokwephula izindonga zeseli.

Q: Uyini umehluko phakathi kwamakhithi amathathu wokukhipha we-gDNA 4992201/4992202, 4992724/4992725, 4992709/4992710?

I-4992201/4992202 iPlant Genomic DNA Kit isebenzisa indlela esekwe kukholamu edinga i-chloroform yokukhipha. Ilungele ikakhulukazi amasampula ezitshalo ezahlukahlukene, kanye ne-powder eyomile yesitshalo. IHi-DNAsecure Plant Kit nayo isekelwe kwikholomu, kepha asikho isidingo sokukhishwa kwe-phenol / chloroform, okuyenza iphephe futhi ingabi nobuthi. Ifanele izitshalo ezinama-polysaccharides aphezulu nokuqukethwe kwe-polyphenol. I-4992709/4992710 DNAquick Plant System isebenzisa indlela esekelwa uketshezi. Ukukhishwa kwePhenol / chloroform akudingeki futhi. Inqubo yokuhlanzwa ilula futhi iyashesha ngaphandle komkhawulo wamanani okuqala esampula, ngakho-ke abasebenzisi bangalungisa inani ngokuvumelana nezidingo zokuhlola. Usayizi omkhulu wezingcezu ze-gDNA ungatholakala ngesivuno esikhulu.

Yini isivuno esilinganiselwayo se-gDNA kusuka kusampula yegazi engu-1 ml nge-TIANamp Blood DNA Kit?

I-genomic DNA ikhishwe emananini ahlukene amasampuli egazi lomuntu yi-TIANamp Blood DNA Kit. Imiphumela imi kanje. Imiphumela ibalwe njengereferensi kuphela, imiphumela yangempela yokukhipha incike ezimweni zamasampuli.

Q: Ingabe i-4992207/4992208 ne-4992722/4992723 ingasetshenziswa ukukhipha ama-blood clots DNA?

Ukukhishwa kwegazi kwe-DNA kwegazi kungenziwa kusetshenziswa ama-reagents ahlinzekwe kulawa makhithi amabili ngokumane uguqule umthetho olandelwayo uye emyalweni othize wokukhishwa kwegazi kwe-DNA. Ikhophi ethambile ye-clot yegazi ye-DNA protocol ingakhishwa uma ucela.

Q: Lapho usebenzisa i-TIANamp Genomic DNA Kit, ungaziqhekeza kanjani izicubu ezintsha zibe ukumiswa kweseli?

Misa okwesikhashana isampula elisha nge-1 ml PBS, usawoti ojwayelekile noma i-TE buffer. Homogenize Ngokuphelele isampula nge-homogenizer bese uqoqa imvula phansi kwephubhu nge-centrifuging. Lahla amandla angaphezu kwawemvelo, uphinde uvuselele ukugqashuka nge-200 μl buffer GA. Ukuhlanzwa kwe-DNA okulandelayo kungenziwa ngokuya ngemiyalo.

Q: Ungawukhetha kanjani umkhiqizo wokukhishwa kwe-DNA kusuka kumasampula e-plasma, serum kanye ne-fluid fluid?

Ukuhlanzwa kwe-gDNA kumasampula e-plasma, serum kanye ne-fluid fluid, Kunconywa i-TIANamp Micro DNA Kit. Ukuhlanzwa kwegciwane i-gDNA kusuka kumasampula we-serum / plasma, Kunconywa i-TIANamp Virus DNA / RNA Kit. Ukuhlanzwa kwe-gDNA yebhaktheriya kusuka kumasampula we-serum ne-plasma, i-TIANamp Bacteria DNA Kit inconywa (i-lysozyme kufanele ifakwe ibhaktheriya elihle). Kumasampula amathe, i-Hi-Swab DNA Kit ne-TIANamp Bacteria DNA Kit kuyanconywa.

Q: Ungawakhetha kanjani amakhithi okukhishwa kwe-gDNA kusuka kumasampula esikhunta?

I-DNAsecure Plant Kit noma i-DNAquick Plant System inconyelwa ukukhishwa kwefungal genome. Ngokukhishwa kwemvubelo ye-genome, Kunconywa i-TIANamp Yeast DNA Kit (i-lyticase kufanele izilungiselele).

Ikhithi Yokukhishwa Kwama-Cell Free Dna