I-TIANamp Genomic DNA Kit

Ukukhishwa kwe-genomic DNA egazini, amaseli nezicubu zezilwane.

I-TIANamp Genomic DNA Kit isuselwe kubuchwepheshe be-silica ulwelwesi futhi inikela ngohlelo olukhethekile lwe-buffer lokukhishwa kwe-genomic DNA kusuka ezinhlotsheni ezahlukahlukene zamasampula. Ukucubungula okulula kwe-centrifugation kususa ngokuphelele ukungcola kanye ne-enzyme inhibitors. I-DNA ehlanzwe yile kit isivele isilungele ukusetshenziswa ekutholakaleni okuphansi noma kwezinye izinhlelo zokusebenza.

Ikati. Cha	Ukupakisha Usayizi
4992199	Ama-preps angama-50
4992254	Ama-preps angama-200

Thumela i-imeyili kithi

Imininingwane yomkhiqizo

Imibuzo evame ukubuzwa

Amathegi womkhiqizo

Izici

■ Ukuhlanzeka okuphezulu: Ulwelwesi lwe-Silica lususa ukungcola okuningi.
■ Ukusetshenziswa okubanzi: Kufanele izicubu, amaseli, igazi nezinye izinhlobo eziningi zamasampuli.
■ Ukusebenza okusheshayo: IGenomic DNA ingatholakala kungakapheli ihora elilodwa.

Ukucaciswa

Thayipha: Spin ikholomu isuselwe
Isampula nevolumu yokuqala: Igazi eliphelele elingu-100-400 μl eliphuma kwizilwane ezincelisayo, 5-20 μl lonke igazi elivela enyonini noma e-amphibian, 10⁶-10⁷ amaseli akhulile, izicubu ezingama-30 mg
Ukukhiqiza: 3-40 μg
Isikhathi sokusebenza: ~ 1 ihora
Izicelo ezingezansi: Ukuvinjelwa kwe-enzyme digestion, ukuhlaziywa kwe-PCR, ukucisha kweSouthern, ilabhulali ye-DNA, njll.

Amanothi abalulekile

■ Sicela usebenzise amasampula amasha ukuqinisekisa ubuqotho be-gDNA ehlanzekile.
■ Uma inani lokuqala lesampula lidlula ibanga elinconyiwe lekithi, kunconywa ukuthi wenyuse inani leBuffer GA neBuffer GB, bese uhlukanisa ukulayishwa kwekholomu ngezikhathi ezihlukene, noma wenze ukuhlolwa usebenzisa amakholomu amabili we-spin.
■ Ngokugcina isikhathi eside kwe-gDNA, kunconywa ukuthi kugcinwe ku-buffer TE. Sicela uqinisekise ukuthi i-OD₂₆₀/ OD₂₈₀ inani likububanzi obungu-1.7-1.9, futhi ukuhlushwa kwe-gDNA kungu-100-300 ng / μl.
Isivuno se-DNA esivela kumasampula namanani ahlukile:

TIANamp Genomic DNA Kit

Yonke imikhiqizo ingenziwa ngezifiso i-ODM / OEM. Ngemininingwane,sicela uchofoze i-Customized Service (ODM / OEM)

Langaphambilini I-TIANamp Bacteria DNA Kit

Olandelayo: I-TIANamp N96 Igazi le-DNA Kit

Q: I-DNA encane noma ayikho ku-eluent.

A-1 Ukuhlushwa okuphansi kwamaseli noma igciwane kusampula yokuqala -Nothisa ukuhlushwa kwamaseli noma amagciwane.

I-A-2 I-lysis enganele yamasampuli-Amasampula awakaxutshwa kahle ne-lysis buffer. Kuphakanyiswa ukuthi uhlanganise kahle nge-pulse-vortexing izikhathi eziyi-1-2. —I-lysis enganele yeseli ebangelwe ukwehla komsebenzi we-proteinase K. —Ukungalingani kwe-cell lysis noma ukwehla kwamaprotheni ngenxa yesikhathi esanele sokugeza. Kuphakanyiswa ukusika izicubu zibe yizicucu ezincane bese unweba isikhathi sokugeza ukuze ususe yonke insalela ku-lysate.

I-A-3 Ukukhangisa okwanele kwe-DNA. -Ayikho i-ethanol noma amaphesenti aphansi esikhundleni se-100% ethanol engezwe ngaphambi kokuthi i-lysate idluliselwe kukholamu ye-spin.

A-4 Inani le-pH le-elution buffer liphansi kakhulu. - Lungisa i-pH iye phakathi kuka-8.0-8.3.

Q: I-DNA ayisebenzi kahle ekuhlolweni kokuphendula kwe-enzymatic reaction.

I-ethanol eyinsalela ku-eluent.

—Kunensalela yokuwasha i-PW esikhaleni. I-ethanol ingasuswa ngokufaka phakathi ikholamu ye-spin ngemizuzu engu-3-5, bese ifakwa ekamelweni lokushisa noma ku-50 ℃ incubator ye-1-2 min.

Q: Ukucekelwa phansi kwe-DNA

A-1 Isampula akuyona entsha. —Khipha i-DNA eyisampula enhle njengokulawula ukunquma ukuthi ngabe i-DNA esampulweni yehle yini.

Ukwelashwa kwangaphambili okungalungile. —Kudalwa ukugaya okweqile kwe-nitrogen, uketshezi olubuyisa umswakama, noma inani elikhulu kakhulu lesampula.

Q: Ungakwenza kanjani ukwelashwa kwangaphambili kwe-gDNA?

Ama-pretreatment kufanele ahluke kumasampula ahlukile. Ngamasampula ezitshalo, qiniseka ukuthi ugaya kahle i-nitrogen engamanzi. Ngamasampuli ezilwane, homogenate noma gaya kahle i-nitrogen engamanzi. Kumasampuli anezindonga zamaseli okunzima ukugqashuka, njengama-bacterium e-G + nemvubelo, kuphakanyiswa ukusebenzisa i-lysozyme, i-lyticase noma izindlela zemishini yokwephula izindonga zeseli.

Q: Uyini umehluko phakathi kwamakhithi amathathu wokukhipha we-gDNA 4992201/4992202, 4992724/4992725, 4992709/4992710?

I-4992201/4992202 iPlant Genomic DNA Kit isebenzisa indlela esekwe kukholamu edinga i-chloroform yokukhipha. Ilungele ikakhulukazi amasampula ezitshalo ezahlukahlukene, kanye ne-powder eyomile yesitshalo. IHi-DNAsecure Plant Kit nayo isekelwe kwikholomu, kepha asikho isidingo sokukhishwa kwe-phenol / chloroform, okuyenza iphephe futhi ingabi nobuthi. Ifanele izitshalo ezinama-polysaccharides aphezulu nokuqukethwe kwe-polyphenol. I-4992709/4992710 DNAquick Plant System isebenzisa indlela esekelwa uketshezi. Ukukhishwa kwePhenol / chloroform akudingeki futhi. Inqubo yokuhlanzwa ilula futhi iyashesha ngaphandle komkhawulo wamanani okuqala esampula, ngakho-ke abasebenzisi bangalungisa inani ngokuvumelana nezidingo zokuhlola. Usayizi omkhulu wezingcezu ze-gDNA ungatholakala ngesivuno esikhulu.

Yini isivuno esilinganiselwayo se-gDNA kusuka kusampula yegazi engu-1 ml nge-TIANamp Blood DNA Kit?

I-genomic DNA ikhishwe emananini ahlukene amasampuli egazi lomuntu yi-TIANamp Blood DNA Kit. Imiphumela imi kanje. Imiphumela ibalwe njengereferensi kuphela, imiphumela yangempela yokukhipha incike ezimweni zamasampuli.

Q: Ingabe i-4992207/4992208 ne-4992722/4992723 ingasetshenziswa ukukhipha ama-blood clots DNA?

Ukukhishwa kwegazi kwe-DNA kwegazi kungenziwa kusetshenziswa ama-reagents ahlinzekwe kulawa makhithi amabili ngokumane uguqule umthetho olandelwayo uye emyalweni othize wokukhishwa kwegazi kwe-DNA. Ikhophi ethambile ye-clot yegazi ye-DNA protocol ingakhishwa uma ucela.

Q: Lapho usebenzisa i-TIANamp Genomic DNA Kit, ungaziqhekeza kanjani izicubu ezintsha zibe ukumiswa kweseli?

Misa okwesikhashana isampula elisha nge-1 ml PBS, usawoti ojwayelekile noma i-TE buffer. Homogenize Ngokuphelele isampula nge-homogenizer bese uqoqa imvula phansi kwephubhu nge-centrifuging. Lahla amandla angaphezu kwawemvelo, uphinde uvuselele ukugqashuka nge-200 μl buffer GA. Ukuhlanzwa kwe-DNA okulandelayo kungenziwa ngokuya ngemiyalo.

Q: Ungawukhetha kanjani umkhiqizo wokukhishwa kwe-DNA kusuka kumasampula e-plasma, serum kanye ne-fluid fluid?

Ukuhlanzwa kwe-gDNA kumasampula e-plasma, serum kanye ne-fluid fluid, Kunconywa i-TIANamp Micro DNA Kit. Ukuhlanzwa kwegciwane i-gDNA kusuka kumasampula we-serum / plasma, Kunconywa i-TIANamp Virus DNA / RNA Kit. Ukuhlanzwa kwe-gDNA yebhaktheriya kusuka kumasampula we-serum ne-plasma, i-TIANamp Bacteria DNA Kit inconywa (i-lysozyme kufanele ifakwe ibhaktheriya elihle). Kumasampula amathe, i-Hi-Swab DNA Kit ne-TIANamp Bacteria DNA Kit kuyanconywa.

Q: Ungawakhetha kanjani amakhithi okukhishwa kwe-gDNA kusuka kumasampula esikhunta?

I-DNAsecure Plant Kit noma i-DNAquick Plant System inconyelwa ukukhishwa kwefungal genome. Ngokukhishwa kwemvubelo ye-genome, Kunconywa i-TIANamp Yeast DNA Kit (i-lyticase kufanele izilungiselele).

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