I-TIANprep N96 Plasmid Kit

Ukuqapha kwangempela kwesikhathi kwesimo se-lysis nokukhishwa okuphezulu kwe-plasmids ephezulu yokuhlanzeka

Ulwelwesi lwe-N96 Plate CP3 kule kit lisebenzisa into entsha ye-silica matrix eyenziwe ngokukhethekile yi-TIANGEN, ekwazi ukukhangisa kahle futhi ngokukhethekile i-DNA ye-plasmid, isuse amaprotheni nokunye ukungcola ngezinga elikhulu kakhulu, ngaleyo ndlela kuqinisekiswe ubumsulwa be-plasmid ekhishiwe. Kungacutshungulwa kuze kufike ku-1.3 ml isiko lebhaktheriya le-plasmid elinekhophi ephezulu, kungasetshenzwa kahle kuwo wonke umthombo, lapho kungakhishwa ngokushesha okungafika ku-5-10 μg we-plasmid DNA (isivuno sihlobene nezinto ezifana nenombolo yekhophi ye-plasmid, isimo sebhaktheriya, njll. ). I-Plasmid DNA ekhishwe yikhithi ingasetshenziswa ekuhlolweni kwe-biology yamangqamuzana njengokuvimbela ukugaya i-enzyme, ukulandelana, ukuhlolwa kwelabhulali, i-ligation kanye noguquko.

Ikati. Cha	Ukupakisha Usayizi
4992892	4 preps
4992893	Ama-preps angama-24

Thumela i-imeyili kithi

Imininingwane yomkhiqizo

Isibonelo sokuhlola

Amathegi womkhiqizo

Izici

■ Isivinini esisheshayo sokukhipha.
■ Ifakwe i-reagent eyingqayizivele ye-TIANRed, ingakhomba ngokusobala izinga le-lysis lesampula enqubeni yokukhipha ukuqinisekisa ukusebenza kahle okukhulu nokuhlanzeka okuphezulu kwe-DNA ye-plasmid.
■ Ukuhlanzeka okuphezulu: I-plasmid DNA ehlanziwe ingasetshenziswa ngqo ekuhlolweni kwehla nomfula.
■ Leli khithi lingasetshenziswa eziteshini zokusebenza okukhishwa kuzo okuzenzakalelayo okuphezulu okuzenzakalelayo.

TIANRed Umbala inkomba

TIANRed Color indicator:

Yonke imikhiqizo ingenziwa ngezifiso i-ODM / OEM. Ngemininingwane,sicela uchofoze i-Customized Service (ODM / OEM)

Langaphambilini I-TIANamp FFPE DNA Kit

Olandelayo: I-TIANamp Igazi le-DNA Midi Kit

	I-Plasmid DNA ikhishwe ku-1 ml womhluzi wobusuku obukhulisiwe we-Escherichia coli (TOP10) (i-LB medium) kusetshenziswa i-TIANprep Highpure N96 Plasmid Kit, kanye nesilinganiso esingu-5-6 μg sokuhlanzeka okuphezulu kwe-pBS plasmid. I-plasmid yalayishwa emthonjeni ngamunye wejeli ye-agarose engu-1%, futhi yatholwa yi-electrophoresis
	I-Plasmid DNA ikhishwe ku-1 ml womhluzi wobusuku obukhulisiwe we-Escherichia coli (TOP10) (i-LB medium) kusetshenziswa i-TIANprep Highpure N96 Plasmid Kit, kanye nesilinganiso esingu-5-6 μg sokuhlanzeka okuphezulu kwe-pBS plasmid. I-Vector: i-pBS; Uhlobo lwePlasmid: TOP10. I-plasmid ilandelwe yi-ABI. I-PRISM 3730XL DNA sequencer.