I-TRNzol Universal Reagent

Ifomula entsha yokuthuthukisa yokuguquguquka kwesampula ebanzi.

I-TRNzol Universal Reagent ingasetshenziselwa ukuhlukanisa i-RNA ephelele kumasampula afana negciwane, amabhaktheriya, ukhunta, isilwane, izicubu zezitshalo noketshezi lomzimba ngekhono le-lysis elingcono nokuzwela okuphezulu. Igcina ubuqotho be-RNA ngenkathi iphazamisa amaseli nokuqedwa kwezinto zeseli ngesikhathi sesampula homogenization. I-RNA ehlukaniswe nalo mkhiqizo ingasebenza ngqo njengamathempulethi wokutholwa okuphansi noma ezinye izinhlelo zokusebenza.

Ikati. Cha	Ukupakisha Usayizi
4992730	100 ml

Thumela i-imeyili kithi

Imininingwane yomkhiqizo

Isibonelo sokuhlola

Imibuzo evame ukubuzwa

Amathegi womkhiqizo

Izici

■ Ukuguquguquka okuphezulu: Uhla lwasendle lokuqalisa ivolumu yesampula, elungele ukukhishwa kwesampula yevolumu enkulu ekuphenduleni okukodwa.
■ Isivuno esikhulu: Indlela yokwehlisa ikhulisa isivuno se-RNA kusampula.
■ Ukusetshenziswa okubanzi: Kufanele amasampula amaningi ahlukene njengezicubu zezitshalo nezilwane, amaseli akhulile, igazi, uketshezi lomzimba, njll.
■ Ukusebenza okusheshayo: IGenomic DNA ingatholakala kungakapheli ihora elilodwa ..

Ukucaciswa

Uhlobo: Ukuzikisa kususelwe
Isampula: Igciwane, amabhaktheriya, ukhunta, isilwane, izicubu zezitshalo, amaseli akhulile kanye noketshezi lomzimba.
Ithagethi: I-RNA
Isikhathi sokusebenza: ~ 1 ihora
Izicelo: I-reagent ye-TRNzol Universal inciphisa ukungcoliswa kokungcola okufana ne-DNA namaprotheni ku-RNA ephelele ehlanziwe, futhi ingasetshenziswa ngqo ekuhlolweni kwe-biology yamangqamuzana anjengeNorthern Blot, iDot Blot, ukuhlolwa kwePolyA, ukuhumusha kwe-vitro, ukuhlaziywa kweRNase yokuvikela, ukwakhiwa kwelabhulali ye-cDNA , I-RT-PCR, i-PCR yesikhathi sangempela nokulandelana okuphezulu.

Yonke imikhiqizo ingenziwa ngezifiso i-ODM / OEM. Ngemininingwane,sicela uchofoze i-Customized Service (ODM / OEM)

Langaphambilini I-RNAprep Pure Micro Kit

Olandelayo: Ikhithi ye-RNAprep Pure Cell / Bacteria

Workflow

Indlela: Izicubu zesibindi segundane le-30 mg, amaqabunga elayisi ayi-100 mg aqoqwa ngokugaya i-liquid nitrogen; 1 × 10⁶Amaseli akhulisiwe we-HepG2 kanye ne-700 μl Saccharomyces Cerevisiae culture medium (OD600 = 0.9) aqoqwe yi-centrifugation. 1 ml we-TRNzol Universal Reagent ovela e-TIANGEN nemikhiqizo efanelekile evela kumphakeli u-L no-T yengezwe kwi-aliquot ngayinye yesampula futhi ukukhishwa kwe-RNA kwenziwa kulandela imigomo enikezwe ngumhlinzeki ngamunye. Umthamo we-elution wawungu-80 μl, 50 μl, 30 μl no-30 μl wamasampuli amane ngokulandelana. I-3 μl ye-eluate ilayishwe kulayini ngamunye.
I-MIII: I-TIANGEN Marker III;
I-electrophoresis yenziwa ngo-6 V / cm ngemizuzu engama-30 ku-1% agarose.
Imiphumela: I-TIANGEN TRNzol Universal Reagent ingakhipha ubumsulwa obuphakeme nobuqotho obuhle be-RNA kusuka esibindini segundane, amaqabunga erayisi, amaseli akhulisiwe namasampuli emvubelo, ngokusebenza kahle okukhulu. Ikhwalithi ye-RNA ifana noma iphakeme kancane kunaleyo yomhlinzeki we-L no-T imikhiqizo.

Q: Ukuvinjelwa kwekholomu

I-A-1 Cell lysis noma i-homogenization ayanele

- Nciphisa ukusetshenziswa kwesampula, khulisa inani le-lysis buffer, khulisa i-homogenization nesikhathi se-lysis.

Inani elingu-A-2 lesampuli likhulu kakhulu

---- Yehlisa inani lesampula elisetshenzisiwe noma wandise inani le-lysis buffer.

Q: Isivuno se-RNA esiphansi

I-A-1 Inganele i-lysis yeseli noma i-homogenization

- Nciphisa ukusetshenziswa kwesampula, khulisa inani le-lysis buffer, khulisa i-homogenization nesikhathi se-lysis.

Inani elingu-A-2 lesampuli likhulu kakhulu

---- Sicela ubheke amandla wokucubungula aphezulu.

I-A-3 RNA ayikhishwa ngokuphelele kukholamu

---- Ngemuva kokungeza amanzi e-RNase-Free, yiyeke imizuzu embalwa ngaphambi kwe-centrifuging.

I-A-4 Ethanol ku-eluent

---- Ngemuva kokuhlanza, i-centrifuge futhi bese ususa i-buffer yokuwasha ngangokunokwenzeka.

I-A-5 Cell culture medium ayisuswanga ngokuphelele

- Lapho uqoqa amaseli, sicela uqiniseke ukuthi ususa okuphakathi kwesiko ngangokunokwenzeka.

A-6 Amaseli agcinwe ku-RNAstore awasebenzi kahle nge-centrifuged

---- Ukuxinana kwe-RNAstore kukhulu kunesilinganiso esiphakathi sesiko leseli; ngakho-ke amandla e-centrifugal kufanele akhuliswe. Kuphakanyiswa ku-centrifuge ku-3000x g.

Okuqukethwe kwe-A-7 okuphansi kwe-RNA nobuningi kusampula

---- Sebenzisa isampula elihle ukuthola ukuthi isivuno esiphansi sidalwa yisampula.

Q: Ukucekelwa phansi kwe-RNA

A-1 Indaba ayisiyintsha

Izicubu ezintsha kufanele zigcinwe ku-nitrogen ewuketshezi ngokushesha noma ngokushesha zifakwe kwi-reagent ye-RNAstore ukuqinisekisa umphumela wokukhishwa.

Inani elingu-A-2 lesampuli likhulu kakhulu

---- Yehlisa inani lesampula.

I-A-3 RNase contamination

- Yize i-buffer enikezwe kukhithi ingenayo i-RNase, kulula ukungcolisa i-RNase ngesikhathi senqubo yokukhipha futhi kufanele iphathwe ngokunakekela.

Ukungcola kwe-A-4 Electrophoresis

---- Faka esikhundleni se-electrophoresis buffer futhi uqiniseke ukuthi izinto ezisetshenziswayo ne-Loading Buffer azinakho ukungcoliswa yi-RNase.

A-5 Kulayishwa kakhulu i-electrophoresis

---- Yehlisa inani lokulayishwa kwesampula, ukulayishwa komthombo ngamunye akufanele kudlule ku-2 μg.

Umbuzo: Ukungcola kwe-DNA

Inani elingu-1 lesampuli likhulu kakhulu

---- Yehlisa inani lesampula.

A-2 Amanye amasampula anokuqukethwe okuphezulu kwe-DNA futhi angalashwa nge-DNase.

---- Enza ukwelashwa kwe-RNase-Free DNase kusixazululo esitholakele se-RNA, futhi i-RNA ingasetshenziswa ngqo ekuhlolweni okulandelayo ngemuva kokwelashwa, noma ingaqhubeka ihlanzwe ngamakhithi wokuhlanzwa we-RNA.

Q: Ungayisusa kanjani i-RNase kokudliwayo kokuhlola kanye nama-glasswares?

Okwezingilazi, kubhakwe ku-150 ° C ngehora elingu-4. Kwiziqukathi zepulasitiki, ucwiliswe ku-0.5 M NaOH ngemizuzu eyi-10, bese ugeza kahle ngamanzi angenawo i-RNase bese uvala inzalo ukususa i-RNase ngokuphelele. Ama-reagents noma izixazululo ezisetshenziswe ekuhlolweni, ikakhulukazi amanzi, kumele zingabi ne-RNase. Sebenzisa amanzi angenawo ama-RNase kuwo wonke amalungiselelo e-reagent (engeza amanzi ebhodleleni lengilazi elihlanzekile, engeza i-DEPC ekugxileni kokugcina okungu-0.1% (V / V), unyakazise ubusuku kanye ne-autoclave).

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