I-TIANGEN inikeze ukuhlolwa okungaphezulu kwezigidi eziyi-100 kwe-ASFV
Lesi sixazululo singasetshenziselwa ukuqapha izifo ngenkathi kuzaliswa izingulube nokutholwa kwegciwane ngesikhathi sokugcinwa kwengulube nokucutshungulwa.
Singanikeza ngezixazululo ezahlukahlukene zokuthola izicubu, igazi, indle, futhi
njengamasampula emvelo.
Ukulondolozwa Kwesampula
Isampula yokwelashwa kwangaphambili
Ukukhishwa Kwe-Nucleic Acid
Isixazululo se-RT-qPCR
I-Oral Swab Isibonelo Sokulondolozwa Kwesikhashana
I-RNAstore Reagent
Vikela i-RNA ngokungafrizi
Isicelo: Ukugcinwa kobuchopho, inhliziyo, izinso, ubende, isibindi, amaphaphu kanye ne-thymus, njll.
Igcina i-RNA: usuku olungu-37 ° C, izinsuku eziyi-7 ngo-15-25 ° C, noma amasonto amane ngo-2-8 ° C. Isitoreji sesikhathi eside ku- -20 ° C noma -80 ° C.
Izicubu isampula ukugaya
Isixazululo se-homogenization
Izicubu Sample Ukugaya
Kukufanele ukukhishwa kwe-DNA / RNA / protein kusuka ezicutshini zezitshalo / zezilwane, inhlabathi, indle, isikhunta, njll.
Lokushisa Ukusebenza: njengoba low -10 ℃
Ukufakwa: amasampula angu-1-24
Umphumela we-RNA yezilwane ekhishwe ngemuva kokugaywa yi-H24R
Inani lesampula: 20 mg (inhliziyo: 10mg) Isampula: Umaka weCrucian carp: I-DNA MarkerIII, TIANGEN, MD103-02, RNA Extraction Kit: I-RNA Easy Fast Tissue / Cell Kit (TIANGEN, 4992732) I-RNA ekhishwe ngemuva kokugaywa yi-TGrinder H24R lokuhlushwa okuphezulu nobumsulwa.
Uchungechunge lwe-Nucleic Acid Extractor oluzenzakalelayo
● Iziteshi ezingama-32- kanye nezingu-96 ozikhethela zona.
● Ukukhishwa okusheshayo kwegciwane le-nucleic acid kungakapheli imizuzu engama-30.
● Amakhithi we-reagent wekhwalithi ephezulu ayatholakala ukuze asebenze kahle.
Isixazululo Sokuvula Okuphezulu
● Ukuhambisana okuphezulu, ukufana okuphelele nesithako esivamile se-nucleic acid emakethe.
● Ukupakisha okwenziwe ngokwezifiso nezinsizakalo ze-OEM zenzelwe ngokwezidingo ezithile.
● Iyahambisana: KingFisher, Hamilton, Beckman Coulter, Chemagen njll.
Isixazululo Sokukhishwa Kwemanuwali
I-TIANamp Virus DNA / RNA Kit
● Kudingeka imishini elula kuphela ukuqedela ukuhlolwa kokukhishwa
● Izinto ezisetshenziswayo zigcwele ngokwehlukana ukugwema ukungcoliswa okuphambene
● Isikhathi esifushane sokukhipha nokusebenza okulula, ngokusebenza kahle okuphezulu.
● Ukupakisha okwenziwe ngokwezifiso nezinsizakalo ze-OEM zenzelwe ngokwezidingo ezithile.
● Ama-enzyme aphezulu abucayi e-RT, e-qPCR ahlinzekwa ngohlelo olwenziwe ngcono lwe-reaction buffer.
● Ukugcwala okuphansi kwezifanekiso kungabonakala ngokunembile.
● Ukupakisha okwenziwe ngokwezifiso nezinsizakalo ze-OEM zenzelwe ngokwezidingo ezithile.
I-Probe-based real time PCR reagent / enzyme eluhlaza / i-ODM / i-OEM
Isistimu Yemipayipi Ezenzakalelayo
Okufakwayo: iziteshi ezilodwa noma ezi-8
Isicelo: PCR noma qPCR ukusetha ukusabela
