I-RNAstore Reagent

I-reagent engabandisi ukuvikela ubuqotho besampula i-RNA.

I-RNAstore Reagent iyi-reagent yokulondolozwa kwezicubu ezingamanzi. Ingena ngokushesha kumaseli wezicubu futhi ivikele amaseli angafriziwe kusuka ku-RNA in situ ngokuvimbela umsebenzi we-RNase kahle, ikwenze kufaneleke kakhulu ekuhlaziyeni ukuphrinta kwezakhi zofuzo.
Ekugcinweni kwesampula yezicubu, izicubu zingacwiliswa ngokushesha eRNAstore ukuze kugcinwe ukonakala kwe-RNA, ukuze isampuli kungadingeki ukuthi icutshungulwe ngokushesha noma iqandiswe ku-nitrogen ewuketshezi.
I-RNAstore Reagent ingasetshenziswa kabanzi kumasampula e-vertebrate ahlukahlukene, kufaka phakathi ubuchopho, inhliziyo, izinso, ubende, isibindi, amaphaphu kanye ne-thymus.

Ikati. Cha Ukupakisha Usayizi
4992727 100 ml

Imininingwane yomkhiqizo

Isibonelo sokuhlola

Amathegi womkhiqizo

Izici

■ Izimo zokugcina: Le kit ingagcinwa isonto elilodwa ekamelweni lokushisa, usuku olungu-37 ku-37 and, futhi okungenani inyanga e-1 ngo-4 ℃. Ngamasampuli wezicubu, cwilisa ku-4 ℃ ubusuku bonke, bese udlulisela ku -20 ℃ noma -80 ℃ ukuze ugcine isikhathi eside.
■ Ukuqandisa okuphindiwe nokuncibilikiswa: Izicubu ezibandayo ku- -20 ℃ noma -80 ℃ zingabanjiswa iqhwa izikhathi ezingama-20 ngaphandle kokuthinta ikhwalithi yokukhishwa kwe-RNA.
■ Izicelo ezisezansi: Ngemuva kokukhipha isampula ku-RNAstore Reagent, inani eliphelele le-RNA lingakhishwa yi-TIANGEN's TRNzol, RNAprep Pure, RNAsimple reagents and kits.

Amanothi abalulekile

■ I-RNAstore ilungele kuphela amasampula amasha wezicubu.
■ I-RNAstore ayizifanele amasampula ezicubu zezitshalo.
The Inani lenani lamasampuli wezicubu kanye ne-RNAstore Reagent kufanele okungenani libe yi-1: 10 (isib. Nge-100 mg tissue, kudingeka okungenani i-1 ml ye-RNAstore).
The Ubukhulu bohlangothi ngalunye lwesampula akufanele budlule ku-0.5 cm ukuqinisekisa ukuthi i-RNAstore ingangena ngokushesha ezicutshini.

Yonke imikhiqizo ingenziwa ngezifiso i-ODM / OEM. Ngemininingwane,sicela uchofoze i-Customized Service (ODM / OEM)


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  • Olandelayo:

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    Experimental Example Izinto: 15 mg Isikhumba sezicubu zesibindi
    Indlela: 0.5 g rat izicubu zesibindi (ezigcinwe ku-RNAstore Reagent) zigcinwe kuma-37 ℃, igumbi lokushisa kanye no-4 ℃ ngokulandelana. Ingqikithi ye-RNA evela kumasampula wesicubu sesibindi sesisindo segundane elingu-15 mg egcinwe emazingeni okushisa ahlukene ikhishwe yodwa kusetshenziswa i-TRNzol Reagent (Cat. No. 4992730).
    Imiphumela: Sicela ubheke isithombe esingenhla se-agarose gel electrophoresis.
    I-2-4 μl yama-100 μl eluates alayishwe kulayini ngamunye.
    C (ukulawula okuhle): isampula lezicubu eligcinwe ngqo ku- -80 ℃.
    I-electrophoresis yenziwa ngo-6 V / cm ngemizuzu engama-30 ku-1% agarose.
    Experimental Example Izinto: 15 mg Isikhumba sezicubu zesibindi
    Indlela: Izicubu zesibindi segreyimu engu-0.5 g (ezigcinwe ku-RNAstore Reagent) zifriziwe izikhathi ezingama-5, 10, 15 nezingama-20 ngokulandelana. Ingqikithi ye-RNA evela kumasampula wesicubu sesibindi samathambo wesibindi ayi-15 mg aqandiswe ngezikhathi ezahlukahlukene ahlukaniswa kusetshenziswa i-TRNzol Reagent (Cat. No. 4992730).
    Imiphumela: Sicela ubheke isithombe esingenhla se-agarose gel electrophoresis. I-2-4 μl yama-100 μl eluates alayishwe kulayini ngamunye.
    C (ukulawula okuhle): isampula lezicubu eligcinwe ngqo ku- -80 ℃.
    5, 10, 15, 20: izikhathi ezibandayo zamasampuli.
    I-electrophoresis yenziwa ngo-6 V / cm ngemizuzu engama-30 ku-1% agarose.
    Bhala umyalezo wakho lapha bese uwuthumela kithi