Ikhithi ye-FastKing One Step RT-qPCR

Isinyathelo esisodwa se-Real-Time RT-PCR ene-SYBR-Green.

I-FastKing One Step RT-qPCR Kit yenzelwe i-Real-Time One Step RT-qPCR ngokusebenzisa i-SYBR Green I. Ikhithi ingasetshenziswa ngesikhathi sangempela sesinyathelo esisodwa i-RT-qPCR yezinhloso ze-RNA, ngokubhalwa phansi kwe-reverse ne-PCR kwenzeka ngokulandelana kushubhu efanayo. Ngakho-ke ukusebenza kulula, futhi kunciphisa ubungozi bokungcola.

I-25 × RT-PCR Enzyme Mix kule kit iyisiqalo se-TIANGEN's novel reverse transcriptase (FastKing RTase), i-anti-anti-modified hotstart Isi-Taq I-DNA polymerase ne-RNase Inhibitor. I-FastKing RTase wuhlobo olusha lwe-molecular modified reverse transcriptase. Ngesici se-hydrophobic esingezwe ngokukhethekile, esinobuhlobo obuqinile be-RNA nokuqina kokushisa. I-FastKing RTase ithuthukise ukusebenza kahle kokubhalwa phansi okuguquguqukayo kanye nekhono lokunweba ithempulethi ye-RNA ngesakhiwo esiyinkimbinkimbi sesibili. Uhlobo olusha lwe-hotstartIsi-Taq I-DNA polymerase esebenza kahle kakhulu isetshenziswa kwinqubo ye-PCR, okwenza ukusabela kwe-PCR ngemuva kokubhalwa phansi kwe-reverse kube nokusebenza okuphezulu kokukhulisa nokucaciswa. Ngaphezu kwalokho, i-2 × FastKing RT-qPCR Buffer (SYBR Green) kulo mkhiqizo uhlelo olusha lokuphendula olwenzelwe ngokukhethekile ama-enzyme amabili angenhla, kufaka phakathi izinto ezidingekayo ze-ionic, ama-dNTP, udayi ohlaza okotshani we-SYBR, isinyathelo esisodwa sokuqinisa kanye nesithuthukisi, ingaqinisekisa ukuthi i-FastKing RTase ne-hotstart entshaIsi-Taq I-DNA polymerase ingadlala indima ephumelela kunazo zonke enqubweni eyodwa yokuphendula okukodwa.

Le kit inikeza ama-curve ajwayelekile ajwayelekile ezindaweni ezahlukahlukene zokulinganisa. Ingakwazi ukuthola ngokunembile nangokubala izinhlobonhlobo zezakhi zofuzo eziphakeme kakhulu neziphansi ezinokuphindeka okuhle nokwethenjelwa okuphezulu.