I-HRM Analysis Kit (i-EvaGreen)

Izici

■ Ukulungiswa okuphezulu: Yamukela odayi abagcwele be-EvaGreen, obunokulungiswa okuphezulu kokuncibilika kwejika esimweni esigcwele futhi okungahlukanisa ukwahluka kwesisekelo esisodwa.
■ Ukucaciswa okuphezulu: Sebenzisa i-anti-anti-modified hotstart DNA polymerase ukunciphisa ukukhulisa okungacacisi futhi uthuthukise ukucaciswa.
■ Ukuzinza okuphezulu: Uhlelo lweBuffer olwenziwe ngokucophelela lwandisa ukuqina kwejika lokuncibilika futhi lithuthukise ukuthembeka kwemiphumela.
■ Ukulungiswa kwe-ROX: Idayi ye-ROX ihlanganiswe ngokwahlukana, eguquguquka kalula ukuyisebenzisa futhi ibe nemiphumela enembile.

Ukucaciswa

Uhlobo: I-Antibody modified Taq DNA polymerase, i-EvaGreen.
Izicelo: Ukuthayipha okwaziwayo kwe-SNP; Ukuhlolwa kwe-SNP okungaziwa; Ukuskena izakhi zofuzo ezingaziwa; Ukuhlaziywa kweMethylation PCR.

Yonke imikhiqizo ingenziwa ngezifiso i-ODM / OEM. Ngemininingwane,sicela uchofoze i-Customized Service (ODM / OEM)

×

IGenomic DNA ikhishwe ku-100 μl wesampula yegazi lomuntu kusetshenziswa i-TIANamp Blood DNA Kit. I-50 ng DNA yalayishwa ngesikhathi sangempela sokutholwa kwe-PCR. Ngokomtapo wezincwadi we-NCBI SNP, i-SNP eyaziwa nge-FEN1 gene (RS 174538) iyakhethwa futhi itholwe kusetshenziswa iRoche LightCycle480. Imiphumela imi kanje:

Imiphumela ikhombisa ukuthi i-HRM Analysis Kit iyindlela ekahle yokuhlaziya indawo ye-SNP enokuphindeka okuphezulu kokuncibilika kwejika le-genotype efanayo, ukucaciswa okucacile kwama-genotypes ahlukile futhi akukho ukwahlulelwa okungalungile.

Imininingwane ye-SNP: …… CGGAAGAACACGTCG [A / G] CAGGAGCAGGCGCCT …… (Imvamisa ye-Allele: A = 0.314, G = 0.686)

I-Primer: Ye-108 bp fragment (FEN1_F: CCTCAACGCTCTCACCATTTTG; FEN1_R: GGCACTTCCTTTTCCGGTTGTG)