Ubuchwepheshe bokuxilongwa kwamangqamuzana, ikakhulukazi ukutholwa kwe-pathogen metagenomic (mNGS), kunamathemba amahle wokufaka isicelo ekuhlolweni kwe-pathogen yendabuko, ukuhlonza okungaziwa okusha kwe-pathogen, ukuxilongwa kokutheleleka okuhlangene, ukuxilongwa kokumelana nezidakamizwa, ukuhlolwa kokuphendula kwabantu nokuhlola ukusebenza ngempumelelo kokutheleleka. Inikeza izindlela ezibalulekile zokuvikela nokulawulwa kwezifo ezithathelwanayo, futhi ikhuthaza ukwenziwa ngcono kwekhono lokuxilonga lomtholampilo.
I-mNGS ingahlaziya ngokuningiliziwe i-microbial kanye ne-host material (i-DNA ne-RNA) kumasampuli eziguli, futhi idlulise kancane kancane isuke elabhorethri iye ekusetshenzisweni komtholampilo, njengengxenye yesisekelo sokuxilongwa kokwehluka kodokotela, futhi inikeze ukusekelwa kokuxilongwa okunenhloso.
I-TIANGEN inikeza isisombululo sokwakhiwa kwe-microorganism yasekuqaleni nokwakhiwa kwelabhulali ye-mNGS.
Ukuphendula ubhadane lwe-COVID-19
Kusukela kwaqhamuka i-COVID-19, i-TIANGEN ihlinzeke ngezigidi ezi-5 zokuhlolwa njengezinto zokusetshenziswa ze-virus nucleic acid extraction kanye ne-fluorescent reagents reagents yabakhiqizi abangaphezulu kwama-200 abathola ama-reagent kanye namayunithi wokuthola emazweni ase-Asia, eNyakatho naseNingizimu Melika, kanye naseYurophu. Futhi kusiza izigidi zabantu emazweni angaphezu kwama-30 emhlabeni wonke.
Imikhiqizo yokukhishwa kwegciwane le-TIANGEN, njengezinto zokusetshenziswa, yabonwa embikweni wokuhlola wokusetshenziswa okuphuthumayo kwe-COVID-19 ekhishwe yi-World Health Organisation ngoJuni 2020, yabalwa ohlwini olunconywayo lwama-reagents amasha wokuthola i-COVID-19 akhishwe yi Isikhwama Somhlaba Wonke ngoJanuwari 2021.
Kusuka kokungahluziwe ukuqeda imikhiqizo
zonke izixhumanisi zilawulwa ngokuqinile ngaphansi kohlelo lokuphathwa kwekhwalithi le-ISO13485
Imvelo Yokukhiqiza
Ama-Raw material
Imikhiqizo engemuva
Isekelwe ku-QC-NGS
Ukulondolozwa Kwesampula
Isampula yokwelashwa kwangaphambili
Ukukhishwa Kwe-Nucleic Acid
NGS
Isampula yomlomo we-swab Preservation Buffer
I-RNAstore Reagent
Vikela i-RNA ngokungafrizi
Isicelo: ubuchopho, inhliziyo, izinso, ubende, isibindi, amaphaphu kanye ne-thymus, njll.
Igcina i-RNA: usuku olungu-37 ° C, izinsuku eziyi-7 ngo-15-25 ° C, noma amasonto amane ngo-2-8 ° C. -20 ° C noma -80 ° C
Izicubu isampula ukugaya
Isixazululo se-homogenization
Kukufanele ukukhishwa kwe-DNA / RNA / protein kusuka ezicutshini zezitshalo / zezilwane, inhlabathi, indle, isikhunta, njll.
Lokushisa Ukusebenza: njengoba low -10 ℃
Ukufakwa: amasampula angu-1-24
Umphumela we-RNA yezilwane ekhishwe ngemuva kokugaywa yi-H24R
Inani lesampula: 20 mg (inhliziyo: 10mg) Isampula: Umaka weCrucian carp: I-DNA MarkerIII, TIANGEN, MD103-02, RNA Extraction Kit: I-RNA Easy Fast Tissue / Cell Kit (TIANGEN, 4992732) I-RNA ekhishwe ngemuva kokugaywa yi-TGrinder H24R lokuhlushwa okuphezulu nobumsulwa.
Uchungechunge lwe-Nucleic Acid Extractor oluzenzakalelayo
● Iziteshi ezingama-32- kanye nezingu-96 ozikhethela zona.
● Ukukhishwa okusheshayo kwegciwane le-nucleic acid kungakapheli imizuzu engama-30.
● Amakhithi we-reagent wekhwalithi ephezulu ayatholakala ukuze asebenze kahle.
Isixazululo Sokuvula Okuphezulu
I-Magnetic Hi-DNA / RNA Kit (4992408 -T4A)
● Ukuhambisana okuphezulu, ukufana okuphelele nesithako esivamile se-nucleic acid emakethe.
● Ukupakisha okwenziwe ngokwezifiso nezinsizakalo ze-OEM zenzelwe ngokwezidingo ezithile.
● Iyahambisana: KingFisher, Hamilton, Beckman Coulter, Chemagen njll.
Isikhathi sangempela se-PCR amplifi cation curve ye-TIANGEN 4992408
Isikhathi sangempela se-PCR amplifi cation curve soMhlinzeki T
I-AIV-H5 yahlanjululwa yaba yi-10-6 -10-8ama-gradients anamanzi we-Milli-Q, bese ekhishwa yi-King Fisher Flex. Kusetshenziswe ama-200 μl esampula ngayinye. I-TIANGEN kit ikhombise ukuzwela okuhle nokusimama. Ithuluzi le-PCR langempela: I-reagent ye-ABI 7500 Real-time PCR: FP314
Isixazululo Sokukhishwa Kwemanuwali
● Kudingeka imishini elula kuphela ukuqedela ukuhlolwa kokukhishwa
● Izinto ezisetshenziswayo zigcwele ngokwehlukana ukugwema ukungcoliswa okuphambene
● Isikhathi esifushane sokukhipha nokusebenza okulula, ngokusebenza kahle okuphezulu.
● Ukupakisha okwenziwe ngokwezifiso nezinsizakalo ze-OEM zenzelwe ngokwezidingo ezithile.
I-TIANamp Virus DNA / RNA Kit (4992285)
I-TIANamp Virus RNA Kit (4992286)
I-TIANamp Micro DNA Kit (4992287)
| Ikati.no. | Uhlobo lwe-Nucleic acid | Uhlobo lwesampula olusetshenziswayo |
| 4992285 | I-DNA / RNA | I-serum, i-plasma, uketshezi lomzimba, izicubu, isisombululo sokulondolozwa kweswabi, isiko lesiko lamagciwane, njll |
| 4992286 | I-RNA | I-serum, i-plasma, uketshezi lomzimba, izicubu, isisombululo sokulondolozwa kweswabi, isiko lesiko lamagciwane, njll |
| 4992287 | I-DNA | Amagciwane, isikhunta, ama-parasites kanye nama-virus ahlukaniswe ne-serum, i-plasma, izicubu, i-pleural ne-ascites, i-cerebrospinal fluid, isikhwehlela, i-bronchoalveolar lavage fluid ne-parafini |
● I-TIANSeq DirectFast Library Kit (i-illumina) (4992259/4992260)
Ukuhlukaniswa kwe-Enzymatic DNA. Ifanele ukwakhiwa kwelabhulali ye-DNA yesikhulumi sokulandelana okuphezulu se-Illumina
● I-TIANSeq Fast DNA Library Kit (i-illumina) (4992261/4992262)
Ifanele ukwakhiwa kwelabhulali ye-DNA yesikhulumi sokulandelana okuphezulu se-Illumina
● Fast Kit Library Library for Ion Torrent Platform (ngokwezifiso kit)
Ifanele ukwakhiwa kwelabhulali ye-DNA yesikhulumi sokulandelana okuphezulu se-Ion torrent
● Fast Kit Library Library ye-MGI Platform (ngokwezifiso kit)
Ifanele ukwakhiwa kwelabhulali ye-DNA yesikhulumi sokulandelana okuphezulu se-MGI
● I-TIANSeq rRNA Depletion Kit (H / M / R) (4992363/4992364/4992391) (yamapulatifomu e-Illumina / Ion torrent / MGI)
Ukususwa kwe-rRNA yomsingathi ukuthuthukisa ukuzwela kokutholwa kwe-RNA yegciwane
● I-TIANSeq Fast RNA Library Prep Kit (Illumina) (4992375)
Okwakhiwa kwelabhulali ye-RNA yegciwane ngemuva kokususa i-rRNA yomsingathi, enganquma ngokushesha ubukhona bokulandelana kwegciwane le-RNA
● I-TIANSeq Stranded RNA-Seq Kit (Illumina) (4993007)
Ngokwakhiwa kwelabhulali ye-RNA yegciwane ngemuva kokususa i-rRNA yomsingathi, enganquma ngokunembile umehluko wokulandelana kwegciwane
● I-RNA RNA Library Prep Kit ye-MGI Platform (Ikhithi eyenziwe ngokwezifiso)
Ifanele ukwakhiwa komtapo wolwazi we-RNA wepulatifomu yokulandelela okuphezulu ye-MGI
● I-TIANSeq Usayizi Ukukhethwa kwe-DNA Ubuhlalu (4992358/4992359/4992979)
Ngokuhlanzwa nokukhethwa kukasayizi wezingcezwana ze-DNA ngesikhathi kwakhiwa umtapo wolwazi we-DNA
● I-TIANSeq RNA Ubuhlalu obuhlanzekile (4992360/4992362/4992867)
Ngokuhlanzwa kwe-RNA ngemuva kokunothisa i-RNA
● I-TIANSeq Adapter-Index Adapter (Illumina) (4992642/4992378)
● I-TIANSeq Dual-Index Adapter (Illumina) (NG216-T1 / 2/3/4/5/6)
Okwakhiwa kwelabhulali ye-DNA yesikhulumi sokulandelana okuphezulu se-Illumina
