RNA Easy Fast Tissue/Cell Kit Featured Image

I-RNA Easy Tissue / Cell Kit

Ukuhlanzwa kwe-RNA esezingeni eliphelele kwizicubu / amaseli ezilwane.

I-RNA Easy Tissue / Cell Kit iyikhithi esheshayo yokukhipha i-RNA yezicubu zezilwane / amasampula amaseli. It is athuthukiswe okusekelwe genomic DNA ukususwa ubuchwepheshe kuphela lakhiwa TIANGEN. Inani elikhulu lamasampuli ahlukene lingacutshungulwa ngasikhathi sinye ngenqubo esheshayo kungakapheli imizuzu engama-30. I-RNA ehlukaniswe nalo mkhiqizo ingasebenza ngqo njengamathempulethi wokutholwa okuphansi noma ezinye izinhlelo zokusebenza.

Ikati. Cha	Ukupakisha Usayizi
4992732	Ama-preps angama-50

Thumela i-imeyili kithi

Imininingwane yomkhiqizo

Isibonelo sokuhlola

Imibuzo evame ukubuzwa

Amathegi womkhiqizo

Izici

■ Ukusebenza okusheshayo: I-RNA ingatholakala kungakapheli imizuzu engama-30.
■ Ukuhlanzeka okuphezulu: Ulwelwesi lwe-Silica lususa ukungcola okuningi futhi ikholomu yokususa i-gDNA isusa i-genomic DNA.
■ Ukusetshenziswa okubanzi: Kufanele amasampula ahlukene njengezicubu, amaseli namagciwane.

Ukucaciswa

Uhlobo: Spin ikholomu isuselwe
Isampula nevolumu yokuqala: 10-20 mg izicubu noma <10⁷ amaseli
Ithagethi: I-RNA
Isikhathi sokusebenza: ~ 30 amaminithi
Izinhlelo zokusebenza ezisezansi: I-RT-PCR / RT-qPCR, i-Northern Blot, i-Dot Blot, i-poly (A), i-in vitro translation, i-RNase protection assay, i-molecular cloning, njll.

Yonke imikhiqizo ingenziwa ngezifiso i-ODM / OEM. Ngemininingwane,sicela uchofoze i-Customized Service (ODM / OEM)

Langaphambilini I-RNAprep Pure Hi-Blood Kit

Olandelayo: I-RNA Easy Plant Tissue Kit

I-RNA ekhishwe kwisampula yesibindi segundane le-15 mg kusetshenziswa i-RNA Easy Fast Tissue / Cell Kit kanye nomkhiqizo ofanele kusuka kumhlinzeki u-A no-T.
Umthamo we-Elution: 100 μl; Ilayisha ivolumu: 3 μl
M: Umaka we-TIANGEN D15000
Umphumela wokuhlola: I-RNA Easy Fast Tissue / Cell Kit inezinga eliphakeme lokukhipha kunomkhiqizo ofanele ovela kumhlinzeki u-A no-T.

Q: Ukuvinjelwa kwekholomu

I-A-1 Cell lysis noma i-homogenization ayanele

- Nciphisa ukusetshenziswa kwesampula, khulisa inani le-lysis buffer, khulisa i-homogenization nesikhathi se-lysis.

Inani elingu-A-2 lesampuli likhulu kakhulu

---- Yehlisa inani lesampula elisetshenzisiwe noma wandise inani le-lysis buffer.

Q: Isivuno se-RNA esiphansi

I-A-1 Inganele i-lysis yeseli noma i-homogenization

- Nciphisa ukusetshenziswa kwesampula, khulisa inani le-lysis buffer, khulisa i-homogenization nesikhathi se-lysis.

Inani elingu-A-2 lesampuli likhulu kakhulu

---- Sicela ubheke amandla wokucubungula aphezulu.

I-A-3 RNA ayikhishwa ngokuphelele kukholamu

---- Ngemuva kokungeza amanzi e-RNase-Free, yiyeke imizuzu embalwa ngaphambi kwe-centrifuging.

I-A-4 Ethanol ku-eluent

---- Ngemuva kokuhlanza, i-centrifuge futhi bese ususa i-buffer yokuwasha ngangokunokwenzeka.

I-A-5 Cell culture medium ayisuswanga ngokuphelele

- Lapho uqoqa amaseli, sicela uqiniseke ukuthi ususa okuphakathi kwesiko ngangokunokwenzeka.

A-6 Amaseli agcinwe ku-RNAstore awasebenzi kahle nge-centrifuged

---- Ukuxinana kwe-RNAstore kukhulu kunesilinganiso esiphakathi sesiko leseli; ngakho-ke amandla e-centrifugal kufanele akhuliswe. Kuphakanyiswa ku-centrifuge ku-3000x g.

Okuqukethwe kwe-A-7 okuphansi kwe-RNA nobuningi kusampula

---- Sebenzisa isampula elihle ukuthola ukuthi isivuno esiphansi sidalwa yisampula.

Q: Ukucekelwa phansi kwe-RNA

A-1 Indaba ayisiyintsha

Izicubu ezintsha kufanele zigcinwe ku-nitrogen ewuketshezi ngokushesha noma ngokushesha zifakwe kwi-reagent ye-RNAstore ukuqinisekisa umphumela wokukhishwa.

Inani elingu-A-2 lesampuli likhulu kakhulu

---- Yehlisa inani lesampula.

I-A-3 RNase contamination

- Yize i-buffer enikezwe kukhithi ingenayo i-RNase, kulula ukungcolisa i-RNase ngesikhathi senqubo yokukhipha futhi kufanele iphathwe ngokunakekela.

Ukungcola kwe-A-4 Electrophoresis

---- Faka esikhundleni se-electrophoresis buffer futhi uqiniseke ukuthi izinto ezisetshenziswayo ne-Loading Buffer azinakho ukungcoliswa yi-RNase.

A-5 Kulayishwa kakhulu i-electrophoresis

---- Yehlisa inani lokulayishwa kwesampula, ukulayishwa komthombo ngamunye akufanele kudlule ku-2 μg.

Umbuzo: Ukungcola kwe-DNA

Inani elingu-1 lesampuli likhulu kakhulu

---- Yehlisa inani lesampula.

A-2 Amanye amasampula anokuqukethwe okuphezulu kwe-DNA futhi angalashwa nge-DNase.

---- Enza ukwelashwa kwe-RNase-Free DNase kusixazululo esitholakele se-RNA, futhi i-RNA ingasetshenziswa ngqo ekuhlolweni okulandelayo ngemuva kokwelashwa, noma ingaqhubeka ihlanzwe ngamakhithi wokuhlanzwa we-RNA.

Q: Ungayisusa kanjani i-RNase kokudliwayo kokuhlola kanye nama-glasswares?

Okwezingilazi, kubhakwe ku-150 ° C ngehora elingu-4. Kwiziqukathi zepulasitiki, ucwiliswe ku-0.5 M NaOH ngemizuzu eyi-10, bese ugeza kahle ngamanzi angenawo i-RNase bese uvala inzalo ukususa i-RNase ngokuphelele. Ama-reagents noma izixazululo ezisetshenziswe ekuhlolweni, ikakhulukazi amanzi, kumele zingabi ne-RNase. Sebenzisa amanzi angenawo ama-RNase kuwo wonke amalungiselelo e-reagent (engeza amanzi ebhodleleni lengilazi elihlanzekile, engeza i-DEPC ekugxileni kokugcina okungu-0.1% (V / V), unyakazise ubusuku kanye ne-autoclave).

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