Talent qPCR PreMix (SYBR Green)

Ukumelana okuhle nokuphazamiseka kokungcola kanye ne-quantification esheshayo yezifanekiso eziyinkimbinkimbi.

Lo mkhiqizo uyi-reagent ekhethekile ye-Real-Time PCR esebenzisa i-SYBR Green I chimeric fluorescence. I-2 × Talent qPCR PreMix isebenzisa i-anti Taq DNA polymerase ye-anti-modified, futhi uhlelo oluyingqayizivele lwe-qPCR Buffer lungaqinisekisa ukuthi umkhiqizo wenza ukusabela okubucayi nokushesha kakhulu kwe-qPCR kuzo zonke izinsimbi ze-Real-Time PCR. Isikhathi sokuphendula se-qPCR singancishiswa ngama-50%. Ngaphezu kwalokho, ukwengezwa kwesici se-H-Competitor ne-EP ku-Buffer kwenza umkhiqizo ube nobubanzi besampula jikelele. Inokusebenza okuhle kakhulu kumathempulethi anezakhiwo eziyinkimbinkimbi ezithuthukile, izifanekiso ezinensalela eningi ye-PCR inhibitor, i-fragment amplification ende, njll.
Umkhiqizo futhi unezici zesilinganiso sokukhulisa okuphezulu, ukucaciswa okuphezulu kokukhulisa, ububanzi obunokwethenjelwa, njll, futhi ungathola imiphumela ngokushesha ngaphandle kokuthinta umphumela we-PCR.

Ikati. Cha	Ukupakisha Usayizi
4992882	20 ×l × 125 rxn
4992775	20 ×l × 500 rxn
4992866	20 ×l × 5000 rxn

Thumela i-imeyili kithi

Imininingwane yomkhiqizo

Isibonelo sokuhlola

Amathegi womkhiqizo

Izici

■ I-anti-polymerase ebucayi kakhulu ye-antibody inganciphisa isikhathi sokuphendula ngesigamu.
■ I-H-Competitor incintisana ne-hydrogen bond, eqinisekisa ukusebenza kahle okuphezulu kwamathempulethi anokuqukethwe okuphezulu kwe-GC, ukwakheka okuyinkimbinkimbi okuyisisekelo namathempulethi amade.
Isakhi se-EP sizinzisa uhlelo lwe-PCR, sivikele ngempumelelo umsebenzi we-enzyme futhi simelane nokuphazamiseka kwama-inhibitors ahlukahlukene.
■ Ishubhu engenambala engenambala ithatha indawo yeshubhu ensundu, ukuze inani elisele libonakale.

Izicelo

Kukulungele ukuhlaziywa kwenkulumo, ukutholwa kwe-nucleic acid nezinye izinhlobo zokuhlolwa kuzinsimbi ezahlukahlukene zesikhathi sangempela se-PCR ngendlela ye-SYBR Green, ikakhulukazi i-GC ephezulu, isakhiwo esiyinkimbinkimbi sesibili, izinsalela zokungcola okuphezulu kanye nokulinganisa kwethempulethi ende.

Yonke imikhiqizo ingenziwa ngezifiso i-ODM / OEM. Ngemininingwane,sicela uchofoze i-Customized Service (ODM / OEM)

Langaphambilini I-FastFire qPCR PreMix (Ukuhlola)

Olandelayo: I-HRM Analysis Kit (i-EvaGreen)

Experimental Example

Uhlobo lwe-Human UH11 (GC: 73.4%) lalinganiswa yi-TIANGEN Talent qPCR PreMix (kwesobunxele) nomkhiqizo ofanele ovela kuMhlinzeki A (maphakathi) kanye no-B (kwesokudla). Imiphumela ikhombisa ukuthi uma kuqhathaniswa nabanye abahlinzeki, i-Talent qPCR PreMix inamajika acacile wokulinganisa ama-gradients ama-5 wokuhlushwa, inani eliphansi le-Ct, inani eliphakeme le-fluorescence, ne-single melting curve peak, okukhombisa ukuthi i-Talent qPCR PreMix inokuzivumelanisa okuhle nokusebenza kahle kwe-templates eziphezulu ze-GC.

Bhala umyalezo wakho lapha bese uwuthumela kithi