I-TGuide FFPE DNA One-Step Kit

Isinyathelo esisodwa sokukhipha i-genome DNA kusuka kumasampula e-FFPE.

I-TGuide FFPE DNA One-Step Kit yenzelwe ngokukhethekile ukukhipha i-genome DNA kumasampula wezicubu zikaphalafini nge-TGuide automated nucleic acid extractors. Leli khithi lamukela indlela yokushisa yesinyathelo esisodwa, ukuze i-deparaffinization kanye ne-lysis yezicubu ingenziwa ngasikhathi sinye, futhi ama-reagents ayingozi njenge-xylene awanalo. Le kit inezinketho ezimbili zokukhipha: Ngamanani amancane amasampuli, khetha amahora amabili we-lysis; Ngamanani amakhulu amasampuli, khetha amahora ayi-16 ngobusuku obubodwa. Ikhithi isebenzisa i-cellulose efakwe ebuchosheni bobuchwepheshe bobuchwepheshe ukukhipha kahle i-genome DNA.

Ikati. Cha	Ukupakisha Usayizi
I-OSR-M405	48 preps

Thumela i-imeyili kithi

Imininingwane yomkhiqizo

Isibonelo sokuhlola

Imibuzo evame ukubuzwa

Amathegi womkhiqizo

Izicelo

I-DNA ehlanzekile ingasetshenziswa ngqo emisebenzini ehlukahlukene ejwayelekile, kufaka phakathi ukugaya kwe-enzymatic, i-PCR, ukwakhiwa kwelabhulali, i-Southern blot nokunye ukuhlolwa.

Izici

■ Ukwehliswa kwemishini ngaphakathi emishinini: Izinyathelo zokususa kanye nokukhipha zingaqedelwa ngokuzenzakalela ngokumane ufake amasampula asetshenzisiwe kaphalafini angene kwikhathriji ye-reagent bese ufaka iSula Oil neProteinase K.
■ Imiphumela enokwethenjelwa: I-genome DNA etholakele ayinakho ukungcoliswa yi-RNA namaprotheni, futhi ingasetshenziswa ngqo kwi-PCR noma i-fluorescence PCit quantitative PCR.
■ Kuphephile futhi akunangozi: Amakhemikhali awuketshezi angokwemvelo ayingozi emzimbeni womuntu njenge phenol chloroform awadingeki.

Yonke imikhiqizo ingenziwa ngezifiso i-ODM / OEM. Ngemininingwane,sicela uchofoze i-Customized Service (ODM / OEM)

Langaphambilini Amaseli e-TGuide / Tissue Genomic DNA Kit

Olandelayo: I-TGuide Bacteria Genomic DNA Kit

Imiphumela ikhombise ukuthi kumasampula amakhulu evolumu, isivuno sokukhishwa kwalezi zindlela ezimbili besingaqhathaniswa; ngamasampuli anezinga eliphakathi nelincane, isivuno sendlela yokuncishiswa kwesinyathelo esisodwa (eyenziwa nge-TGuide) sasisikhulu kunaleso sendlela evamile yokwehliswa kwepharafini. Ngakho-ke, indlela yokuncishiswa kwesinyathelo esisodwa ayenzi lula ukusebenza kuphela, inciphisa ubungozi, kepha futhi ithuthukisa ukusebenza kahle kwesizinda.
Qaphela:
Isampula yevolumu enkulu: indawo yokuhlukanisa ingaphakathi kuka-2.5-4 cm² futhi ubukhulu buphakathi kuka-5-20 μm.
Isampula yevolumu emaphakathi: indawo yokuhlukanisa ingaphakathi kuka-1.5-2.5 cm² futhi ubukhulu buphakathi kuka-5-20 μm.
Isampula yevolumu encane: indawo yokuhlukanisa ingaphakathi kuka-0.2-1.5 cm² futhi ubukhulu buphakathi kuka-5-20 μm.

Q: I-DNA encane noma ayikho ku-eluent.

A-1 Ukuhlushwa okuphansi kwamaseli noma igciwane kusampula yokuqala -Nothisa ukuhlushwa kwamaseli noma amagciwane.

I-A-2 I-lysis enganele yamasampuli-Amasampula awakaxutshwa kahle ne-lysis buffer. Kuphakanyiswa ukuthi uhlanganise kahle nge-pulse-vortexing izikhathi eziyi-1-2. —I-lysis enganele yeseli ebangelwe ukwehla komsebenzi we-proteinase K. —Ukungalingani kwe-cell lysis noma ukwehla kwamaprotheni ngenxa yesikhathi esanele sokugeza. Kuphakanyiswa ukusika izicubu zibe yizicucu ezincane bese unweba isikhathi sokugeza ukuze ususe yonke insalela ku-lysate.

I-A-3 Ukukhangisa okwanele kwe-DNA. -Ayikho i-ethanol noma amaphesenti aphansi esikhundleni se-100% ethanol engezwe ngaphambi kokuthi i-lysate idluliselwe kukholamu ye-spin.

A-4 Inani le-pH le-elution buffer liphansi kakhulu. - Lungisa i-pH iye phakathi kuka-8.0-8.3.

Q: I-DNA ayisebenzi kahle ekuhlolweni kokuphendula kwe-enzymatic reaction.

I-ethanol eyinsalela ku-eluent.

—Kunensalela yokuwasha i-PW esikhaleni. I-ethanol ingasuswa ngokufaka phakathi ikholamu ye-spin ngemizuzu engu-3-5, bese ifakwa ekamelweni lokushisa noma ku-50 ℃ incubator ye-1-2 min.

Q: Ukucekelwa phansi kwe-DNA

A-1 Isampula akuyona entsha. —Khipha i-DNA eyisampula enhle njengokulawula ukunquma ukuthi ngabe i-DNA esampulweni yehle yini.

Ukwelashwa kwangaphambili okungalungile. —Kudalwa ukugaya okweqile kwe-nitrogen, uketshezi olubuyisa umswakama, noma inani elikhulu kakhulu lesampula.

Q: Ungakwenza kanjani ukwelashwa kwangaphambili kwe-gDNA?

Ama-pretreatment kufanele ahluke kumasampula ahlukile. Ngamasampula ezitshalo, qiniseka ukuthi ugaya kahle i-nitrogen engamanzi. Ngamasampuli ezilwane, homogenate noma gaya kahle i-nitrogen engamanzi. Kumasampuli anezindonga zamaseli okunzima ukugqashuka, njengama-bacterium e-G + nemvubelo, kuphakanyiswa ukusebenzisa i-lysozyme, i-lyticase noma izindlela zemishini yokwephula izindonga zeseli.

Q: Uyini umehluko phakathi kwamakhithi amathathu wokukhipha we-gDNA 4992201/4992202, 4992724/4992725, 4992709/4992710?

I-4992201/4992202 iPlant Genomic DNA Kit isebenzisa indlela esekwe kukholamu edinga i-chloroform yokukhipha. Ilungele ikakhulukazi amasampula ezitshalo ezahlukahlukene, kanye ne-powder eyomile yesitshalo. IHi-DNAsecure Plant Kit nayo isekelwe kwikholomu, kepha asikho isidingo sokukhishwa kwe-phenol / chloroform, okuyenza iphephe futhi ingabi nobuthi. Ifanele izitshalo ezinama-polysaccharides aphezulu nokuqukethwe kwe-polyphenol. I-4992709/4992710 DNAquick Plant System isebenzisa indlela esekelwa uketshezi. Ukukhishwa kwePhenol / chloroform akudingeki futhi. Inqubo yokuhlanzwa ilula futhi iyashesha ngaphandle komkhawulo wamanani okuqala esampula, ngakho-ke abasebenzisi bangalungisa inani ngokuvumelana nezidingo zokuhlola. Usayizi omkhulu wezingcezu ze-gDNA ungatholakala ngesivuno esikhulu.

Yini isivuno esilinganiselwayo se-gDNA kusuka kusampula yegazi engu-1 ml nge-TIANamp Blood DNA Kit?

I-genomic DNA ikhishwe emananini ahlukene amasampuli egazi lomuntu yi-TIANamp Blood DNA Kit. Imiphumela imi kanje. Imiphumela ibalwe njengereferensi kuphela, imiphumela yangempela yokukhipha incike ezimweni zamasampuli.

Q: Ingabe i-4992207/4992208 ne-4992722/4992723 ingasetshenziswa ukukhipha ama-blood clots DNA?

Ukukhishwa kwegazi kwe-DNA kwegazi kungenziwa kusetshenziswa ama-reagents ahlinzekwe kulawa makhithi amabili ngokumane uguqule umthetho olandelwayo uye emyalweni othize wokukhishwa kwegazi kwe-DNA. Ikhophi ethambile ye-clot yegazi ye-DNA protocol ingakhishwa uma ucela.

Q: Lapho usebenzisa i-TIANamp Genomic DNA Kit, ungaziqhekeza kanjani izicubu ezintsha zibe ukumiswa kweseli?

Misa okwesikhashana isampula elisha nge-1 ml PBS, usawoti ojwayelekile noma i-TE buffer. Homogenize Ngokuphelele isampula nge-homogenizer bese uqoqa imvula phansi kwephubhu nge-centrifuging. Lahla amandla angaphezu kwawemvelo, uphinde uvuselele ukugqashuka nge-200 μl buffer GA. Ukuhlanzwa kwe-DNA okulandelayo kungenziwa ngokuya ngemiyalo.

Q: Ungawukhetha kanjani umkhiqizo wokukhishwa kwe-DNA kusuka kumasampula e-plasma, serum kanye ne-fluid fluid?

Ukuhlanzwa kwe-gDNA kumasampula e-plasma, serum kanye ne-fluid fluid, Kunconywa i-TIANamp Micro DNA Kit. Ukuhlanzwa kwegciwane i-gDNA kusuka kumasampula we-serum / plasma, Kunconywa i-TIANamp Virus DNA / RNA Kit. Ukuhlanzwa kwe-gDNA yebhaktheriya kusuka kumasampula we-serum ne-plasma, i-TIANamp Bacteria DNA Kit inconywa (i-lysozyme kufanele ifakwe ibhaktheriya elihle). Kumasampula amathe, i-Hi-Swab DNA Kit ne-TIANamp Bacteria DNA Kit kuyanconywa.

Q: Ungawakhetha kanjani amakhithi okukhishwa kwe-gDNA kusuka kumasampula esikhunta?

I-DNAsecure Plant Kit noma i-DNAquick Plant System inconyelwa ukukhishwa kwefungal genome. Ngokukhishwa kwemvubelo ye-genome, Kunconywa i-TIANamp Yeast DNA Kit (i-lyticase kufanele izilungiselele).

Ikhithi Yokukhishwa Kwama-Cell Free Dna