I-TGuide S96 Igazi / Iseli / Izicubu ze-RNA Kit

I-TGuide S96 Igazi / Iseli / Izicubu ze-RNA Kit zisebenzisa ubuhlalu bemagnethi ngomsebenzi owehlukile wokuhlukanisa nohlelo oluyingqayizivele lwe-buffer lokuhlukanisa nokuhlanza i-RNA ephelele esezingeni eliphezulu. Umkhiqizo ungafaniswa ngokuphelele nesikhiphi se-TGuide S96. Ubuhlalu bukaMagnetic buhlanjululwa, budluliswe futhi bukhishwe ngezinduku ezikhethekile zikazibuthe, ngaleyo ndlela kuqaphele ukudluliswa kobuhlalu bamagnetic nama-nucleic acid. Ingqikithi ekhishwe ye-RNA inokuhlanzeka okuphezulu futhi ayinakho ukungcoliswa kwama-genome, amaprotheni nokunye ukungcola.

Ikati. Cha Ukupakisha Usayizi
4992977 96 preps

Imininingwane yomkhiqizo

Imibuzo evame ukubuzwa

Amathegi womkhiqizo

Izici:

■ Kulula futhi kuyashesha: I-RNA enokuhlanzeka okuphezulu ingatholakala kalula ngehora elilodwa.
■ Ukufakwa okuphezulu: amasampula angama-96 angakhishwa nge-TGuide S96 Automated Nucleic Acid Extractor.
Safe Iphephile futhi ayinabo ubuthi: Azikho izidalwa ezinobuthi ezifana ne-phenol / chloroform ezidingekayo.
■ Ukuhlanzeka okuphezulu: I-RNA etholakalayo inokuhlanzeka okuphezulu.

Ukucaciswa

Uhlobo: Ukukhishwa kohlobo lobuhlalu be-Magnetic.
Isampula: Igazi, Iseli, Izicubu.
Ithagethi:  Ingqikithi ye-RNA
Iqala ivolumu: 500 μl
Isikhathi sokusebenza: 60 iminithi
Izinhlelo zokusebenza ezisezansi: I-PCR, ukwakhiwa kwelabhulali ye-NGS, njll.

Yonke imikhiqizo ingenziwa ngezifiso i-ODM / OEM. Ngemininingwane,sicela uchofoze i-Customized Service (ODM / OEM)


  • Langaphambilini
  • Olandelayo:

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    Q: Ukuvinjelwa kwekholomu

    I-A-1 Cell lysis noma i-homogenization ayanele

    - Nciphisa ukusetshenziswa kwesampula, khulisa inani le-lysis buffer, khulisa i-homogenization nesikhathi se-lysis.

    Inani elingu-A-2 lesampuli likhulu kakhulu

    ---- Yehlisa inani lesampula elisetshenzisiwe noma wandise inani le-lysis buffer.

    Q: Isivuno se-RNA esiphansi

    I-A-1 Inganele i-lysis yeseli noma i-homogenization

    - Nciphisa ukusetshenziswa kwesampula, khulisa inani le-lysis buffer, khulisa i-homogenization nesikhathi se-lysis.

    Inani elingu-A-2 lesampuli likhulu kakhulu

    ---- Sicela ubheke amandla wokucubungula aphezulu.

    I-A-3 RNA ayikhishwa ngokuphelele kukholamu

    ---- Ngemuva kokungeza amanzi e-RNase-Free, yiyeke imizuzu embalwa ngaphambi kwe-centrifuging.

    I-A-4 Ethanol ku-eluent

    ---- Ngemuva kokuhlanza, i-centrifuge futhi bese ususa i-buffer yokuwasha ngangokunokwenzeka.

    I-A-5 Cell culture medium ayisuswanga ngokuphelele

    - Lapho uqoqa amaseli, sicela uqiniseke ukuthi ususa okuphakathi kwesiko ngangokunokwenzeka.

    A-6 Amaseli agcinwe ku-RNAstore awasebenzi kahle nge-centrifuged

    ---- Ukuxinana kwe-RNAstore kukhulu kunesilinganiso esiphakathi sesiko leseli; ngakho-ke amandla e-centrifugal kufanele akhuliswe. Kuphakanyiswa ku-centrifuge ku-3000x g.

    Okuqukethwe kwe-A-7 okuphansi kwe-RNA nobuningi kusampula

    ---- Sebenzisa isampula elihle ukuthola ukuthi isivuno esiphansi sidalwa yisampula.

    Q: Ukucekelwa phansi kwe-RNA

    A-1 Indaba ayisiyintsha

    Izicubu ezintsha kufanele zigcinwe ku-nitrogen ewuketshezi ngokushesha noma ngokushesha zifakwe kwi-reagent ye-RNAstore ukuqinisekisa umphumela wokukhishwa.

    Inani elingu-A-2 lesampuli likhulu kakhulu

    ---- Yehlisa inani lesampula.

    I-A-3 RNase contamination

    - Yize i-buffer enikezwe kukhithi ingenayo i-RNase, kulula ukungcolisa i-RNase ngesikhathi senqubo yokukhipha futhi kufanele iphathwe ngokunakekela.

    Ukungcola kwe-A-4 Electrophoresis

    ---- Faka esikhundleni se-electrophoresis buffer futhi uqiniseke ukuthi izinto ezisetshenziswayo ne-Loading Buffer azinakho ukungcoliswa yi-RNase.

    A-5 Kulayishwa kakhulu i-electrophoresis

    ---- Yehlisa inani lokulayishwa kwesampula, ukulayishwa komthombo ngamunye akufanele kudlule ku-2 μg.

    Umbuzo: Ukungcola kwe-DNA

    Inani elingu-1 lesampuli likhulu kakhulu

    ---- Yehlisa inani lesampula.

    A-2 Amanye amasampula anokuqukethwe okuphezulu kwe-DNA futhi angalashwa nge-DNase.

    ---- Enza ukwelashwa kwe-RNase-Free DNase kusixazululo esitholakele se-RNA, futhi i-RNA ingasetshenziswa ngqo ekuhlolweni okulandelayo ngemuva kokwelashwa, noma ingaqhubeka ihlanzwe ngamakhithi wokuhlanzwa we-RNA.

    Q: Ungayisusa kanjani i-RNase kokudliwayo kokuhlola kanye nama-glasswares?

    Okwezingilazi, kubhakwe ku-150 ° C ngehora elingu-4. Kwiziqukathi zepulasitiki, ucwiliswe ku-0.5 M NaOH ngemizuzu eyi-10, bese ugeza kahle ngamanzi angenawo i-RNase bese uvala inzalo ukususa i-RNase ngokuphelele. Ama-reagents noma izixazululo ezisetshenziswe ekuhlolweni, ikakhulukazi amanzi, kumele zingabi ne-RNase. Sebenzisa amanzi angenawo ama-RNase kuwo wonke amalungiselelo e-reagent (engeza amanzi ebhodleleni lengilazi elihlanzekile, engeza i-DEPC ekugxileni kokugcina okungu-0.1% (V / V), unyakazise ubusuku kanye ne-autoclave).

    Bhala umyalezo wakho lapha bese uwuthumela kithi