TIANtough Genotyping qPCR PreMix (Probe) Featured Image

Ngokwe-Genotyping qPCR PreMix (Probe)

Probe reagent ukuthayipha okunembile kwesayithi le-SNP.

I-TIANexact Genotyping qPCR PreMix (Probe) isilungele ukusetshenziswa i-2 × hot-start PCR premix reagent esetshenziselwe ukucubungula-kusekelwe Ukutholwa kwe-SNP. I-reagent ehlelwe ngaphambili iqukethe i-antibody ethile eguquliweIsi-Taq I-DNA polymerase futhi ingazitholela ngempumelelo izifanekiso ze-DNA eziphansi ngokweqile. Isilondolozi se-PCR esilungiswe ngokukhethekile singaqeda ngempumelelo ukuvimbela okuningi ku-PCR kanye nomthelela ekucinyweni kwesiginali ye-fluorescence. Leli khithi linokucaciswa okuhle nokusebenza kahle kokukhulisa ukuhlonza kwe-SNP, ikakhulukazi amasampula ongahluziwe, akhombisa ukumelana nokucindezela okukhulu.

Ikati. Cha	Ukupakisha Usayizi
4992874	20 ×l × 125 rxn
4992875	20 ×l × 500 rxn

Thumela i-imeyili kithi

Imininingwane yomkhiqizo

Isibonelo sokuhlola

Amathegi womkhiqizo

Izici

■ Ukucaciswa okuphezulu: I-anti-specific anti-modified start-start Taq DNA polymerase inciphisa ukukhulisa okungacacisiwe futhi ithuthukise ukucaciswa.
■ I-genotyping esebenzayo: Isistimu ye-Buffer ehlukile ingavikela ngempumelelo ithonya lama-PCR inhibitors ekuthayipheni kwe-SNP.
■ Ukunemba okuphezulu: I-SNP enamandla yokuthayipha amasiginali we-fluorescence yenza imiphumela kube lula ukuyicacisa.
■ Ukusebenza okubanzi: Kusebenza ekutshaleni, ezilwaneni nakwezinye amasampuli, nakho kusebenza ekutholeni okukhishwa okungahluziwe kwamanye amasampula ezemvelo.
■ Inkomba eluhlaza okwesibhakabhaka: I-reagent esandulelwe ngaphambili iqukethe udayi wenkomba eluhlaza okwesibhakabhaka ukugwema isampula lokulayisha amaphutha.
■ Ukulungiswa kwe-ROX: Idayi ye-ROX ihlanganiswe ngokwahlukana, eguquguquka kalula ukuyisebenzisa futhi ibe nemiphumela enembile.

Ukucaciswa

Uhlobo: I-antibody-based antibody modified Taq DNA polymerase
Izicelo: Ukuthayipha okwaziwayo kwe-SNP

Yonke imikhiqizo ingenziwa ngezifiso i-ODM / OEM. Ngemininingwane,sicela uchofoze i-Customized Service (ODM / OEM)

Langaphambilini I-HRM Analysis Kit (i-EvaGreen)

Olandelayo: Umbala weSuperReal PreMix (SYBR Green)

	Uma kuqhathaniswa ne-Competitor I, i-TIANexact ikhombisa isignali enamandla ye-fluorescence nomphumela wokuqina oqinile ngaphansi kwesimo sethempulethi lokuhlushwa eliphansi.
	Lapho welashwa nge-humic acid (50 ng / μl), ukukhuliswa kwe-PCR kweCompetitor I kuvinjelwe ngokuphelele, kuyilapho i-TIANexact isakhombisa imiphumela enembile.