EndoFree Maxi Plasmid Kit Featured Image

Ikhithi ye-EndoFree Maxi Plasmid

Ukuhlanzwa kwe-plasmid DNA ye-endotoxin-free transfection grade.

I-EndoFree Maxi Plasmid Kit isuselwe kubuchwepheshe be-silica ulwelwesi ukukhipha i-endasmxin-free plasmid DNA. Le khithi inikeza inqubo eyingqayizivele yokususa i-endotoxin ukukhiqiza ikhwalithi ephezulu ye-DNA yezinhlelo zokusebenza ezisezansi. Idizayinelwe ukuhlukanisa i-plasmid DNA yokuhlanzeka okuphezulu kakhulu kusuka ku-100-200 ml yesiko lamagciwane futhi ingaveza kuze kufike ku-1.5 mg ibanga lokudluliswa kwebanga le-plasmid DNA.

Ikati. Cha	Ukupakisha Usayizi
4992194	Ama-preps ayi-10

Thumela i-imeyili kithi

Imininingwane yomkhiqizo

Isibonelo sokuhlola

Amathegi womkhiqizo

Izici

■ Isivuno esisheshayo nesiphezulu: 200 μg-1.5 mg plasmid DNA isivuno ehoreni eli-1 ngesilinganiso esiphakeme sesakhiwo esigoqwe kakhulu.
■ Ukuhlanzeka okuphezulu: Ubumsulwa be-plasmid DNA etholakala ngohlelo oluyingqayizivele lwe-buffer ne-Spin Column CP6.
■ Ukusebenza kahle kokungenisa izifo: Kufanele ukuhlolwa kokushintshwa kolayini abaningi beseli.
■ Izinhlobonhlobo zezinhlelo zokusebenza: Ifanele ukugayelwa kwe-enzyme digestion, inguquko, ukulandelana, i-microinjection, ukuthulisa izakhi zofuzo kanye nokuhlolwa kokuhambisa.

Isivuno sePlasmid

Plasmid Yield

Yonke imikhiqizo ingenziwa ngezifiso i-ODM / OEM. Ngemininingwane,sicela uchofoze i-Customized Service (ODM / OEM)

Langaphambilini I-TIANprep Rapid N96 Plasmid Kit

Olandelayo: I-EndoFree Mini Plasmid Kit II

Ikhophi ephezulu / ephansi yekhophi ye-plasmid
I-plasmid pBR322 enekhophi ephansi, ekhishwe kumasiko wamagciwane ayi-200 ml kusetshenziswa i-EndoFree Maxi Plasmid Kit, yathathelwa phezulu ku-1 ml Buffer TB, ene-0.6 μg / μl. Ikhophi ephezulu ye-plasmid pBS, ekhishwe kumasiko wamagciwane ayi-100 ml kusetshenziswa i-EndoFree Maxi Plasmid Kit, yathathwa nge-1 ml Buffer TB, ene-1.2 μg / μl.
I-MIV: I-TIANGEN DNA Umaka IV
i-pBR322: 2 μl, i-pBS: 2 μl

I-pEGFP etholwe nge-EndoFree Maxi Plasmid Kit idluliselwe ngokwehlukana yaba umugqa weseli ongazweli we-endotoxin 293T kanye namaseli weseli azwela i-endotoxin Huh7 amaseli yi-TIANGEN TIANfect. Ukuchazwa kwe-GFP kutholakale emahoreni angama-24 ngemuva kokushintshelwa.

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