I-TIANprep Rapid N96 Plasmid Kit

Ukuhamba okuphezulu, ukukhipha ngokushesha inani elincane le-plasmid.

Ikhithi isebenzisa indlela yezulu ye-isopropanol ukuthola i-plasmid DNA. Ikhithi isebenzisa i-96-well filter plate N ne-Buffer III, ekhiqizwa ngokukhethekile yi-TIANGEN, futhi ingakhipha ngokushesha nangendlela efanele i-DNA ephezulu ye-plasmid. Kufanelekile ukulungiselela okusheshayo kwama-plasmids aphezulu. I-1.3 ml yesiko lamagciwane le-plasmid enekhophi ephezulu ingacutshungulwa emthonjeni ngamunye, futhi amasampula angama-96 e-plasmid DNA akhishwa ngokushesha. Isivuno esiphezulu emthonjeni ngamunye ngu-10-20 μg (isivuno sihlobene nezinto ezifana nenombolo yekhophi ye-plasmid, isimo se-bacterial, njll.). I-Plasmid DNA ekhishwe yikhithi ingasetshenziswa ekuhlolweni kwe-biology yamangqamuzana njengokuvimbela ukugaya i-enzyme, ukulandelana, ukuhlolwa kwelabhulali, i-ligation kanye noguquko.

Ikati. Cha:	Ukupakisha Usayizi
4992868	4 preps
4992869	24 preps

Thumela i-imeyili kithi

Imininingwane yomkhiqizo

Ukuhamba komsebenzi

Isibonelo sokuhlola

Amathegi womkhiqizo

Izici

■ Ilula futhi ilula: Amasampula angama-96 angakhishwa emahoreni ama-2-3.
■ Ifakwe i-reagent eyingqayizivele ye-TIANRed, ingakhomba ngokusobala izinga le-lysis lesampula enqubeni yokukhipha ukuze kuqinisekiswe ubumsulwa be-plasmid DNA ephezulu.
■ Ukuhlanzeka okuphezulu: I-plasmid enobumsulwa obuphakeme ingasetshenziswa ngqo kuzilingo ezisezansi.

Yonke imikhiqizo ingenziwa ngezifiso i-ODM / OEM. Ngemininingwane,sicela uchofoze i-Customized Service (ODM / OEM)

Langaphambilini Ikhithi ye-EndoFree Midi Plasmid

Olandelayo: Ikhithi ye-EndoFree Maxi Plasmid

TIANprep Rapid N96 Plasmid Kit01

	I-Plasmid DNA ikhishwe ku-1 ml womhluzi wobusuku obukhulisiwe we-Escherichia coli (TOP10) (i-LB medium) kusetshenziswa i-TIANprep Rapid N96 Plasmid Kit, kanye nesilinganiso esingu-8-10 μg sokuhlanzeka okuphezulu kwe-pBS plasmid kwatholakala. I-2 l i-plasmid ilayishwe emthonjeni ngamunye we-1% yejeli ye-agarose, yatholwa yi-electrophoresis.
	I-Plasmid DNA ikhishwe ku-1 ml womhluzi wobusuku obukhulisiwe we-Escherichia coli (TOP10) (i-LB medium) kusetshenziswa i-TIANprep Rapid N96 Plasmid Kit, kanye nesilinganiso esingu-8-10 μg sokuhlanzeka okuphezulu kwe-pBS plasmid kwatholakala. I-Vector: i-pBS plasmid; Ubunzima: TOP10. I-plasmid ilandelwe yi-ABI. I-PRISM 3730XL DNA sequencer