I-EndoFree Mini Plasmid Kit II

Ukuhlanzwa kwe-plasmid DNA ye-endotoxin-free transfection grade.

I-EndoFree Mini Plasmid Kit II isebenzisa ubuchwepheshe obuyingqayizivele be-silica membrane adsorption ukuze ibophe kahle futhi ibophele i-DNA ye-plasmid. Uhlelo olukhethekile lokususa i-endotoxin lungakususa ngempumelelo ukungcola okufana ne-endotoxin namaprotheni. Yonke inqubo yokukhipha ithatha ihora elilodwa kuphela, elilula futhi elisheshayo. Isivuno se-plasmid extraction sihlobene nenombolo yekhophi ye-plasmid, uhlobo lwe-host host kanye nezimo zesiko. Kuze kufike ku-70 μg ye-plasmid DNA engakhishwa ku-5-15 ml wesilinganiso esiphakathi kwamasiko amabhaktheriya akhulisiwe.

Ikati. Cha	Ukupakisha Usayizi
4992422	Ama-preps angama-50

Thumela i-imeyili kithi

Imininingwane yomkhiqizo

Isibonelo sokuhlola

Amathegi womkhiqizo

Izici

■ Isivuno esisheshayo nesiphezulu: 5-70 μg plasmid DNA ingakhishwa cishe ehoreni elilodwa.
■ Ukuhlanzeka okuphezulu: Uhlelo olukhethekile lokususa i-endotoxin kuhlanganiswe ne-Spin Column CP4 kungakhangisa ngokukhethekile i-nucleic acid futhi kususe i-endotoxin ezingeni elincane.
■ Izicelo ezahlukahlukene: Zilungele ukuhlolwa okujwayelekile njengokuvinjelwa kwe-endonuclease digestion, i-PCR, ukulandelana, i-ligation, uguquko, kanye nokuhlolwa okunemba okuphezulu okufana nokwelashwa kofuzo, i-cell microinjection, ukuthuliswa kwezakhi zofuzo, ukuloba, njll.

Isivuno sePlasmid

Plasmid Yield

Yonke imikhiqizo ingenziwa ngezifiso i-ODM / OEM. Ngemininingwane,sicela uchofoze i-Customized Service (ODM / OEM)

Langaphambilini Ikhithi ye-EndoFree Maxi Plasmid

Olandelayo: I-TIANprep N96 Magnetic Plasmid Kit

Kufanelekile ukukhishwa kwamaplasmid ekhophi aphezulu / aphansi
Umdwebo 1. Ukukhishwa kwe-DNA ye-plasmid kusuka kumthamo ohlukahlukene wegciwane lesiko usebenzisa i-EndoFree Mini Plasmid Kit II.
Umthamo we-Elution: 200 μl; Ukulayisha ivolumu: 3 μl ikhophi ephansi ye-plasmid pBR322, 2 μl yekhophi ephezulu ye-plasmid pBS.
I-electrophoresis yenziwa ngo-6 V / cm ngemizuzu engama-30 ku-1% agarose.
Ukudluliswa kweseli yi-plasmid ehlanziwe

Umdwebo 2 I-pEGFP plasmid ehlanzwe yi-EndoFree Mini Plasmid Kit II idluliselwe kumaseli angama-293T. Ukuchazwa kwe-GFP kutholwe ku-48 h ngemuva kokuhanjiswa

Bhala umyalezo wakho lapha bese uwuthumela kithi