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I-TIANprep Rapid Mini Plasmid Kit

Ukuhlanzwa okusheshayo kwe-plasmid DNA yebanga lamangqamuzana e-alkaline lysis inqubo.

I-TIANprep Rapid Mini Plasmid Kit yenzelwe ubuchwepheshe be-alkaline lysis bendabuko, lapho i-DNA yekhwalithi ephezulu ingahlanzwa kungakapheli imizuzu eyi-8. I-lysis buffer entsha ivumela ukukhangiswa kwe-DNA kulwelwesi lwe-silica lapho kukhona usawoti omningi. Okuqukethwe okwenziwe ulwelwesi lwe-silica kuhlukile, kusebenza kahle kakhulu futhi kucaciswe kakhulu. Leli kit lenzelwe ukuhlanzwa okungafika kuma-24 μg plasmid DNA kusuka ku-1-4 ml amasiko asebusuku weE.coli. I-plasmid DNA ehlanzwe yileli kit ingasetshenziswa ekuhlolweni okujwayelekile okuhlukahlukene, okufana nokugaywa kwe-enzyme, i-PCR, ukulandelana, ukuhlanganiswa, ukuguqulwa, ukuhlolwa komtapo wezincwadi, ukubhala nge-in vitro nokudluliswa kwamaseli ajwayelekile amasiko, njll.

Ikati. Cha	Ukupakisha Usayizi
4992191	Ama-preps angama-50
4992192	Ama-preps angama-200

Thumela i-imeyili kithi

Imininingwane yomkhiqizo

Ukuhamba komsebenzi

Isibonelo sokuhlola

Amathegi womkhiqizo

Izici

■ Kuyashesha: Inqubo elula enezinyathelo ezimbalwa zokuhlanza i-plasmid DNA emsulwa kumaminithi ayi-8 kuphela.
■ Ukusebenza kahle okuphezulu: Kungahlanza ngaphezu kwe-85% ye-plasmid DNA kusuka ku- E.coli.
■ Isibonisi sombala i-TIANRed sanikezwa ukulawula nokukhumbuza yonke inqubo ngesikhathi sokuhlolwa ukuqinisekisa ukusebenza kahle kokuhlanzwa kwe-plasmid.

Yonke imikhiqizo ingenziwa ngezifiso i-ODM / OEM. Ngemininingwane,sicela uchofoze i-Customized Service (ODM / OEM)

Langaphambilini TGuide S32 Automated Nucleic Acid extractor

Olandelayo: Ikhithi ye-EndoFree Midi Plasmid

Ngokwelashwa okuphezulu kwe-lysate kanye ne-buffer system, iTIANprep Rapid Mini Plasmid Kit ingathola kalula imikhiqizo esezingeni eliphakeme ngenkathi igcina isikhathi esiningi sokusebenza.

Ukuqhathanisa phakathi kwe-TIANprep Rapid Mini Plasmid Kit noMhlinzeki A (i-plasmid DNA purification kit) ngokuhlanza i-plasmid efanayo ngasikhathi sinye.

Ivolumu yesampula: 3 ml isiko lobusuku bonke lika-E.coli (OD600 = 1.8); Umthamo we-Elution: 50 μl; Ukulayisha ivolumu: 1 μl pBS, 3 μl pET30a, 3 μl pBR322.

I-electrophoresis yenziwa ngo-6 V / cm ngemizuzu engama-30 ku-1% ijeli ye-agarose. M: I-TIANGEN DNA Umaka IV.

Isiphetho: I-Agarose gel electrophoresis ikhombisa ukuthi i-TIANprep Rapid Mini Plasmid Kit ingathola isivuno esifanayo se-plasmid DNA njengaleyo yikhithi yokuhlanza i-plasmid engakapheli imizuzu engu-8.

TIANprep Rapid Mini Plasmid Kit