RNAprep Pure Cell/Bacteria Kit Featured Image

Ikhithi ye-RNAprep Pure Cell / Bacteria

Ukuhlanzwa kwe-RNA esezingeni eliphelele kusuka kumaseli nakwigciwane.

I-RNAprep Pure Cell / Bacteria Kit inikeza indlela esheshayo, elula futhi engabizi kakhulu yokuhlanzwa kwe-RNA ephelele kusuka kumaseli akhulisiwe namasampula amabhaktheriya ngokusebenzisa ikholamu esebenzayo ye-spin nohlelo oluyingqayizivele lwe-buffer. Ikhithi ifaka i-RNase-Free Spin Column CR3 yokuhlanza ikhwalithi ephezulu ye-RNA ngokusebenzisa ubuchwepheshe be-silica-membrane. Ikhwalithi ephezulu ye-RNA ingatholakala ngemizuzu engama-30 kuya kwengama-40 ngokuhlanzeka okuphezulu futhi ayinawo amaprotheni kanye ne-genomic DNA contamination.

Ikati. Cha	Ukupakisha Usayizi
4992235	Ama-preps angama-50

Thumela i-imeyili kithi

Imininingwane yomkhiqizo

Isibonelo sokuhlola

Imibuzo evame ukubuzwa

Amathegi womkhiqizo

Izici

Buff Ama-buffers nama-protocols alungiselelwe amaseli akhulile namasampuli amabhaktheriya enza inqubo ibe lula futhi ibe lula.
■ I-DNase Eyingqayizivele I inciphisa ukungcoliswa kwe-genomic DNA.
■ Amakholomu wokuhlunga we-RNase-Free Unique aqeda okunye ukungcola.
■ Ukuhlanzeka okuphezulu nokulungele ukusetshenziswa i-RNA ilungele izinhlelo ezizwelayo ezisemfuleni.
■ Akukho ukukhishwa kwe-phenol / chloroform, ayikho i-LiCl ne-ethanol, akukho kudilizwa kwe-CsCl gradient centrifugation, okwenza inqubo iphephe futhi ithembeke.

Izicelo

■ I-RT-PCR.
■ I-Northern Blot, iDot Blot.
■ I-PCR Yesikhathi Sangempela.
■ Ukuhlaziywa kwe-Chip.
■ Ukuhlolwa kwePolyA, ukuhumusha kwe-in vitro, ukuhlaziywa kokuvikelwa kwe-RNase nokuhlanganiswa kwamangqamuzana.

Yonke imikhiqizo ingenziwa ngezifiso i-ODM / OEM. Ngemininingwane,sicela uchofoze i-Customized Service (ODM / OEM)

Langaphambilini I-TRNzol Universal Reagent

Olandelayo: Ikhithi ye-RNAprep Tissue Pure

	Okubalulekile: Amaseli E-Jurkat Abantu (1 × 10⁶ ) Indlela: Ingqikithi ye-RNA yama-Human Jukat Cell ahlukaniswe kusetshenziswa i-RNAprep Pure Cell / Bacteria Kit. Imiphumela: Sicela ubheke isithombe esingenhla se-agarose gel electrophoresis. I-2-4 μl yama-50 μl eluates alayishwe kulayini ngamunye. I-electrophoresis yenziwa ngo-6 V / cm ngemizuzu engama-30 ku-1% ijeli ye-agarose.
	Izinto: TOP10 E.coli (1 × 10⁸) Indlela: I-RNA ephelele ye-TOP10 E.coli yahlukaniswa kusetshenziswa i-RNAprep Pure Cell / Bacteria Kit. Imiphumela: Sicela ubheke isithombe esingenhla se-agarose gel electrophoresis. I-2-4 μl yama-50 μl eluates alayishwe kulayini ngamunye. I-electrophoresis yenziwa ngo-6 V / cm ngemizuzu engama-30 ku-1% ijeli ye-agarose.

Q: Ukuvinjelwa kwekholomu

I-A-1 Cell lysis noma i-homogenization ayanele

- Nciphisa ukusetshenziswa kwesampula, khulisa inani le-lysis buffer, khulisa i-homogenization nesikhathi se-lysis.

Inani elingu-A-2 lesampuli likhulu kakhulu

---- Yehlisa inani lesampula elisetshenzisiwe noma wandise inani le-lysis buffer.

Q: Isivuno se-RNA esiphansi

I-A-1 Inganele i-lysis yeseli noma i-homogenization

- Nciphisa ukusetshenziswa kwesampula, khulisa inani le-lysis buffer, khulisa i-homogenization nesikhathi se-lysis.

Inani elingu-A-2 lesampuli likhulu kakhulu

---- Sicela ubheke amandla wokucubungula aphezulu.

I-A-3 RNA ayikhishwa ngokuphelele kukholamu

---- Ngemuva kokungeza amanzi e-RNase-Free, yiyeke imizuzu embalwa ngaphambi kwe-centrifuging.

I-A-4 Ethanol ku-eluent

---- Ngemuva kokuhlanza, i-centrifuge futhi bese ususa i-buffer yokuwasha ngangokunokwenzeka.

I-A-5 Cell culture medium ayisuswanga ngokuphelele

- Lapho uqoqa amaseli, sicela uqiniseke ukuthi ususa okuphakathi kwesiko ngangokunokwenzeka.

A-6 Amaseli agcinwe ku-RNAstore awasebenzi kahle nge-centrifuged

---- Ukuxinana kwe-RNAstore kukhulu kunesilinganiso esiphakathi sesiko leseli; ngakho-ke amandla e-centrifugal kufanele akhuliswe. Kuphakanyiswa ku-centrifuge ku-3000x g.

Okuqukethwe kwe-A-7 okuphansi kwe-RNA nobuningi kusampula

---- Sebenzisa isampula elihle ukuthola ukuthi isivuno esiphansi sidalwa yisampula.

Q: Ukucekelwa phansi kwe-RNA

A-1 Indaba ayisiyintsha

Izicubu ezintsha kufanele zigcinwe ku-nitrogen ewuketshezi ngokushesha noma ngokushesha zifakwe kwi-reagent ye-RNAstore ukuqinisekisa umphumela wokukhishwa.

Inani elingu-A-2 lesampuli likhulu kakhulu

---- Yehlisa inani lesampula.

I-A-3 RNase contamination

- Yize i-buffer enikezwe kukhithi ingenayo i-RNase, kulula ukungcolisa i-RNase ngesikhathi senqubo yokukhipha futhi kufanele iphathwe ngokunakekela.

Ukungcola kwe-A-4 Electrophoresis

---- Faka esikhundleni se-electrophoresis buffer futhi uqiniseke ukuthi izinto ezisetshenziswayo ne-Loading Buffer azinakho ukungcoliswa yi-RNase.

A-5 Kulayishwa kakhulu i-electrophoresis

---- Yehlisa inani lokulayishwa kwesampula, ukulayishwa komthombo ngamunye akufanele kudlule ku-2 μg.

Umbuzo: Ukungcola kwe-DNA

Inani elingu-1 lesampuli likhulu kakhulu

---- Yehlisa inani lesampula.

A-2 Amanye amasampula anokuqukethwe okuphezulu kwe-DNA futhi angalashwa nge-DNase.

---- Enza ukwelashwa kwe-RNase-Free DNase kusixazululo esitholakele se-RNA, futhi i-RNA ingasetshenziswa ngqo ekuhlolweni okulandelayo ngemuva kokwelashwa, noma ingaqhubeka ihlanzwe ngamakhithi wokuhlanzwa we-RNA.

Q: Ungayisusa kanjani i-RNase kokudliwayo kokuhlola kanye nama-glasswares?

Okwezingilazi, kubhakwe ku-150 ° C ngehora elingu-4. Kwiziqukathi zepulasitiki, ucwiliswe ku-0.5 M NaOH ngemizuzu eyi-10, bese ugeza kahle ngamanzi angenawo i-RNase bese uvala inzalo ukususa i-RNase ngokuphelele. Ama-reagents noma izixazululo ezisetshenziswe ekuhlolweni, ikakhulukazi amanzi, kumele zingabi ne-RNase. Sebenzisa amanzi angenawo ama-RNase kuwo wonke amalungiselelo e-reagent (engeza amanzi ebhodleleni lengilazi elihlanzekile, engeza i-DEPC ekugxileni kokugcina okungu-0.1% (V / V), unyakazise ubusuku kanye ne-autoclave).

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