■ Izimo zokugcina: Le kit ingagcinwa isonto elilodwa ekamelweni lokushisa, usuku olungu-37 ku-37 and, futhi okungenani inyanga e-1 ngo-4 ℃. Ngamasampuli wezicubu, cwilisa ku-4 ℃ ubusuku bonke, bese udlulisela ku -20 ℃ noma -80 ℃ ukuze ugcine isikhathi eside.
■ Ukuqandisa okuphindiwe nokuncibilikiswa: Izicubu ezibandayo ku- -20 ℃ noma -80 ℃ zingabanjiswa iqhwa izikhathi ezingama-20 ngaphandle kokuthinta ikhwalithi yokukhishwa kwe-RNA.
■ Izicelo ezisezansi: Ngemuva kokukhipha isampula ku-RNAstore Reagent, inani eliphelele le-RNA lingakhishwa yi-TIANGEN's TRNzol, RNAprep Pure, RNAsimple reagents and kits.
■ I-RNAstore ilungele kuphela amasampula amasha wezicubu.
■ I-RNAstore ayizifanele amasampula ezicubu zezitshalo.
The Inani lenani lamasampuli wezicubu kanye ne-RNAstore Reagent kufanele okungenani libe yi-1: 10 (isib. Nge-100 mg tissue, kudingeka okungenani i-1 ml ye-RNAstore).
The Ubukhulu bohlangothi ngalunye lwesampula akufanele budlule ku-0.5 cm ukuqinisekisa ukuthi i-RNAstore ingangena ngokushesha ezicutshini.
Yonke imikhiqizo ingenziwa ngezifiso i-ODM / OEM. Ngemininingwane,sicela uchofoze i-Customized Service (ODM / OEM)
Izinto: 15 mg Isikhumba sezicubu zesibindi Indlela: 0.5 g rat izicubu zesibindi (ezigcinwe ku-RNAstore Reagent) zigcinwe kuma-37 ℃, igumbi lokushisa kanye no-4 ℃ ngokulandelana. Ingqikithi ye-RNA evela kumasampula wesicubu sesibindi sesisindo segundane elingu-15 mg egcinwe emazingeni okushisa ahlukene ikhishwe yodwa kusetshenziswa i-TRNzol Reagent (Cat. No. 4992730). Imiphumela: Sicela ubheke isithombe esingenhla se-agarose gel electrophoresis. I-2-4 μl yama-100 μl eluates alayishwe kulayini ngamunye. C (ukulawula okuhle): isampula lezicubu eligcinwe ngqo ku- -80 ℃. I-electrophoresis yenziwa ngo-6 V / cm ngemizuzu engama-30 ku-1% agarose. |
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Izinto: 15 mg Isikhumba sezicubu zesibindi Indlela: Izicubu zesibindi segreyimu engu-0.5 g (ezigcinwe ku-RNAstore Reagent) zifriziwe izikhathi ezingama-5, 10, 15 nezingama-20 ngokulandelana. Ingqikithi ye-RNA evela kumasampula wesicubu sesibindi samathambo wesibindi ayi-15 mg aqandiswe ngezikhathi ezahlukahlukene ahlukaniswa kusetshenziswa i-TRNzol Reagent (Cat. No. 4992730). Imiphumela: Sicela ubheke isithombe esingenhla se-agarose gel electrophoresis. I-2-4 μl yama-100 μl eluates alayishwe kulayini ngamunye. C (ukulawula okuhle): isampula lezicubu eligcinwe ngqo ku- -80 ℃. 5, 10, 15, 20: izikhathi ezibandayo zamasampuli. I-electrophoresis yenziwa ngo-6 V / cm ngemizuzu engama-30 ku-1% agarose. |
Kusukela yasungulwa, imboni yethu ibilokhu ithuthukisa imikhiqizo esezingeni lomhlaba yokuqala ngokunamathela esimisweni
wekhwalithi kuqala. Imikhiqizo yethu izuze idumela elihle kakhulu embonini futhi ithembekile phakathi kwamakhasimende amasha namadala ..