■ Izinkomba zombala ziyangezwa kuma-reagents ukugwema amaphutha wokungezwa kwesampula noma ukweqiwa ngenxa yenani elikhulu lamasampuli.
■ Umbala weSuperReal PreMix unenani eliphakeme le-fluorescence, ukuzwela okuphezulu nokucaciswa okuphezulu.
■ Kufanele izinqubo eziningi ezivamile ze-fluorescent (FAM, HEX, NED, njll.). Inkomba ngeke ithinte ukukhanya kwe-probe.
■ Kufanele izinhlobonhlobo zezinsimbi ezijwayelekile zesikhathi sangempela se-PCR (iRoche, i-Bio-rad, i-ABI, njll.).
Ifanele izinhlobo ezahlukahlukene zokuhlolwa njengokuhlaziywa kwenkulumo nokutholwa kwe-nucleic acid ngendlela yophenyo kuzinsimbi ezahlukahlukene zesikhathi sangempela se-PCR. Ilungele ikakhulukazi ukuhlolwa okuphezulu okuphumayo ngenani elikhulu lamasampula nokulayishwa okuyisampula okuyinkimbinkimbi.
Yonke imikhiqizo ingenziwa ngezifiso i-ODM / OEM. Ngemininingwane,sicela uchofoze i-Customized Service (ODM / OEM)
Umdwebo 1. Amagrafu amajika wokukhulisa kanye namajika ajwayelekile atholakele ngokulandelana kokulinganisa uhlobo lomuntu lwe-HSUBC kusetshenziswa i-TIANGEN SuperReal PreMix Plus (Probe) (A) ne-SuperReal PreMix Colour (Probe) (B). Isifanekiso sihlanjululwe ama-gradients wokuhlushwa ama-5. Amanani we-Ct (C) akhombisa ukuthi ukukhuliswa kwamakhithi amabili kunokuzwela okukhulu nokuzinza. Ithuluzi elisetshenzisiwe yi-ABI 7500 kanti iqembu le-probe fluorescent yi-FAM.
Kusukela yasungulwa, imboni yethu ibilokhu ithuthukisa imikhiqizo esezingeni lomhlaba yokuqala ngokunamathela esimisweni
wekhwalithi kuqala. Imikhiqizo yethu izuze idumela elihle kakhulu embonini futhi ithembekile phakathi kwamakhasimende amasha namadala ..