Izicubu Zamagnetic / Iseli / Igazi Ingqikithi ye-RNA Kit

Khipha i-RNA kusuka kumasampuli ahlukahlukene njengegazi lezicubu zomzimba elinamandla amakhulu.

I-Magnetic Tissue / Cell / Blood Ingqikithi ye-RNA isebenzisa ubuhlalu bukazibuthe obunomsebenzi owehlukile wokuhlukanisa nohlelo oluyingqayizivele lokuhlukanisa nokuhlanza inani eliphelele le-RNA ephelele. Umkhiqizo ungafaniswa ngokuphelele neKingfisher Flex96 neTGuide S32 / S96 Automated Nucleic Acid Extractors. Uma kudingeka ukukhipha okuzenzakalelayo okuphezulu, sicela uxhumane ne-TIANGEN ukuthola isixazululo sokuhlanganisa.

Ikati. Cha	Ukupakisha Usayizi
4992740	Ama-preps angama-50

Thumela i-imeyili kithi

Imininingwane yomkhiqizo

Izibonelo Zokuhlola

Imibuzo evame ukubuzwa

Amathegi womkhiqizo

Izici

■ Kulula futhi kuyashesha: Inani eliphelele le-Ultrapure RNA lingatholakala kungakapheli imizuzu engama-60.
■ Ukufakwa okuphezulu: Kungakwazi ukuhlangabezana nezidingo zokukhishwa ngesandla kanye nokukhishwa kwe-batch kuzingxenyekazi ezahlukahlukene eziphezulu.
■ Kuphephile futhi akunabuthi: Akukho reagent efana ne-phenol / chloroform edingekayo.

Ukucaciswa

Uhlobo: Isizinda sohlobo lobuhlalu be-Magnetic.
Isampula: Izicubu, amaseli negazi.
Okuqondiwe: Ingqikithi ye-RNA
Ivolumu yokuqala: 5-20 mg, ingeqi ku-1 × 10⁷, 0.1-1.5 ml
Isikhathi sokusebenza: 60 min
Izicelo ezisezansi: I-RT-PCR / RT-qPCR, ukwakhiwa kwelabhulali ye-NGS, njll.

Yonke imikhiqizo ingenziwa ngezifiso i-ODM / OEM. Ngemininingwane,sicela uchofoze i-Customized Service (ODM / OEM)

Langaphambilini I-Magnetic Circulating DNA Maxi Kit V2

Olandelayo: I-TGuide S96 Magnetic Universal DNA Kit

Ingqikithi ye-RNA ikhishwe esibindini segundane lama-20 mg nge-TIANGEN Magnetic Tissue / Cell / Blood Total RNA Kit kanye nemikhiqizo efanelekile evela kwabanye abahlinzeki. I-5 μl ye-200 μl eluate ilayishwe ku-1% agarose gel electrophoresis, 6 V / cm.
Isiphetho: Isivuno sesizinda, ubumsulwa nokuzinza kweTIANGEN Magnetic Tissue / Cell / Blood Total RNA Kit zingcono kunezabanye abahlinzeki.

Ingqikithi ye-RNA ikhishwe ku-20 mg wezinso zamagundane nge-TIANGEN Magnetic Tissue / Cell / Blood Total RNA Kit in TIANGEN TGuide S32 or Thermo Kingfisher Flex96 ngokulandelana. I-5 μl ye-200 μl eluate ilayishwe ku-1% agarose gel electrophoresis, 6 V / cm. Umaka: I-TIANGEN D15000 DNA Marker.
Isiphetho: I-Magnetic Tissue / Cell / Blood Ingqikithi ye-RNA inevuno enhle nokuhlanzeka kuma-extractor ahlukile azenzakalelayo.

Q: Ukuvinjelwa kwekholomu

I-A-1 Cell lysis noma i-homogenization ayanele

- Nciphisa ukusetshenziswa kwesampula, khulisa inani le-lysis buffer, khulisa i-homogenization nesikhathi se-lysis.

Inani elingu-A-2 lesampuli likhulu kakhulu

---- Yehlisa inani lesampula elisetshenzisiwe noma wandise inani le-lysis buffer.

Q: Isivuno se-RNA esiphansi

I-A-1 Inganele i-lysis yeseli noma i-homogenization

- Nciphisa ukusetshenziswa kwesampula, khulisa inani le-lysis buffer, khulisa i-homogenization nesikhathi se-lysis.

Inani elingu-A-2 lesampuli likhulu kakhulu

---- Sicela ubheke amandla wokucubungula aphezulu.

I-A-3 RNA ayikhishwa ngokuphelele kukholamu

---- Ngemuva kokungeza amanzi e-RNase-Free, yiyeke imizuzu embalwa ngaphambi kwe-centrifuging.

I-A-4 Ethanol ku-eluent

---- Ngemuva kokuhlanza, i-centrifuge futhi bese ususa i-buffer yokuwasha ngangokunokwenzeka.

I-A-5 Cell culture medium ayisuswanga ngokuphelele

- Lapho uqoqa amaseli, sicela uqiniseke ukuthi ususa okuphakathi kwesiko ngangokunokwenzeka.

A-6 Amaseli agcinwe ku-RNAstore awasebenzi kahle nge-centrifuged

---- Ukuxinana kwe-RNAstore kukhulu kunesilinganiso esiphakathi sesiko leseli; ngakho-ke amandla e-centrifugal kufanele akhuliswe. Kuphakanyiswa ku-centrifuge ku-3000x g.

Okuqukethwe kwe-A-7 okuphansi kwe-RNA nobuningi kusampula

---- Sebenzisa isampula elihle ukuthola ukuthi isivuno esiphansi sidalwa yisampula.

Q: Ukucekelwa phansi kwe-RNA

A-1 Indaba ayisiyintsha

Izicubu ezintsha kufanele zigcinwe ku-nitrogen ewuketshezi ngokushesha noma ngokushesha zifakwe kwi-reagent ye-RNAstore ukuqinisekisa umphumela wokukhishwa.

Inani elingu-A-2 lesampuli likhulu kakhulu

---- Yehlisa inani lesampula.

I-A-3 RNase contamination

- Yize i-buffer enikezwe kukhithi ingenayo i-RNase, kulula ukungcolisa i-RNase ngesikhathi senqubo yokukhipha futhi kufanele iphathwe ngokunakekela.

Ukungcola kwe-A-4 Electrophoresis

---- Faka esikhundleni se-electrophoresis buffer futhi uqiniseke ukuthi izinto ezisetshenziswayo ne-Loading Buffer azinakho ukungcoliswa yi-RNase.

A-5 Kulayishwa kakhulu i-electrophoresis

---- Yehlisa inani lokulayishwa kwesampula, ukulayishwa komthombo ngamunye akufanele kudlule ku-2 μg.

Umbuzo: Ukungcola kwe-DNA

Inani elingu-1 lesampuli likhulu kakhulu

---- Yehlisa inani lesampula.

A-2 Amanye amasampula anokuqukethwe okuphezulu kwe-DNA futhi angalashwa nge-DNase.

---- Enza ukwelashwa kwe-RNase-Free DNase kusixazululo esitholakele se-RNA, futhi i-RNA ingasetshenziswa ngqo ekuhlolweni okulandelayo ngemuva kokwelashwa, noma ingaqhubeka ihlanzwe ngamakhithi wokuhlanzwa we-RNA.

Q: Ungayisusa kanjani i-RNase kokudliwayo kokuhlola kanye nama-glasswares?

Okwezingilazi, kubhakwe ku-150 ° C ngehora elingu-4. Kwiziqukathi zepulasitiki, ucwiliswe ku-0.5 M NaOH ngemizuzu eyi-10, bese ugeza kahle ngamanzi angenawo i-RNase bese uvala inzalo ukususa i-RNase ngokuphelele. Ama-reagents noma izixazululo ezisetshenziswe ekuhlolweni, ikakhulukazi amanzi, kumele zingabi ne-RNase. Sebenzisa amanzi angenawo ama-RNase kuwo wonke amalungiselelo e-reagent (engeza amanzi ebhodleleni lengilazi elihlanzekile, engeza i-DEPC ekugxileni kokugcina okungu-0.1% (V / V), unyakazise ubusuku kanye ne-autoclave).

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